FB2026_02 , released June 18, 2026
Allele: Hsap\RAC1PA.Q61L.UAS.mCherry,Tag:AsLOV2
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General Information
Symbol
Hsap\RAC1PA.Q61L.UAS.mCherry,Tag:AsLOV2
Species
H. sapiens
Name
FlyBase ID
FBal0243922
Feature type
allele
Associated gene
Hsap\RAC1
Associated Insertion(s)
Carried in Construct
P{UAS-mCherry-PA-RacQ61L}
Also Known As
UAS-mCherry-PA-RacQ61L
Key Links
Transgenic product class
missense_variant
(Wang et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Wang et al., 2010)
Mutagen
in vitro construct
(Wang et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-mCherry-PA-RacQ61L}
Cytology
Description

UAS regulatory sequences drive expression of a mutated form of Hsap\RAC1 (carries a Q61L amino acid replacement) that is tagged at the N-terminal end with mCherry. The coding sequence is also tagged with Tag:AsLOV2, rendering the protein photoactivatable.

(Wang et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsap\RAC1
Tagged with
mCherry
Tag:AsLOV2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Photoactivation of Hsap\RAC1PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 in the centre of a single border cell (where its expression is under the control of Scer\GAL4slbo.2.6) has negligible effect on border cell cluster migration if the photoactivated cell is at the front of the border cell cluster. However, if the photoactivated cell is at the back of the border cell cluster, net cluster movement backwards is seen.

      (Inaki et al., 2012)

      In the absence of illumination migration of border cells expressing Hsap\RAC1PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 under the control of Scer\GAL4slbo.2.6 is unperturbed.

      Photoactivation of Hsap\RAC1PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 at the rear of border cell clusters expressing Hsap\RAC1PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 under the control of Scer\GAL4slbo.2.6 results in reverse border cell migration. When photoactivation is stopped, border cells stall for a variable period of time before regaining forward migration.

      (Wang et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Border cell clusters expressing Rac1N17.Scer\UAS and Hsap\RAC1PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 under the control of Scer\GAL4slbo.2.6 in which Hsap\RAC1PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 has been activated by illumination in one cell do not show forward migration. However, photoactivation of Hsap\RAC1PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 in approximately half of the cluster results in slow forward movement.

      In the absence of illumination, border cells expressing EgfrDN.Scer\UAS and PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 and carrying Hsap\RAC1PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 fail to move forward. When Hsap\RAC1PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 is photoactivated at the rear of the border cell cluster, rearward movement is seen. When photoactivation is stopped, the border cell cluster stops moving.

      (Wang et al., 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Hsap\RAC1PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Asat\PT1
      Hsap\RAC1PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1
      Hsap\RAC1PA.Q61L.UAS.mCherry,Tag:AsLOV2
      Name Synonyms
      Secondary FlyBase IDs
        References (5)