FB2026_02 , released June 18, 2026
Allele: Hsap\RAC1PA.T17N.UAS.mCherry,Tag:AsLOV2
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General Information
Symbol
Hsap\RAC1PA.T17N.UAS.mCherry,Tag:AsLOV2
Species
H. sapiens
Name
FlyBase ID
FBal0243923
Feature type
allele
Associated gene
Hsap\RAC1
Associated Insertion(s)
Carried in Construct
P{UAS-mCherry-PA-RacT17N}
Key Links
Transgenic product class
dominant_negative_variant
(Wang et al., 2010)
missense_variant
(Wang et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Wang et al., 2010)
missense_variant
(Wang et al., 2010)
Mutagen
in vitro construct
(Wang et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-mCherry-PA-RacT17N}
Cytology
Description

UAS regulatory sequences drive expression of a dominant-negative form of Hsap\RAC1 (carries a T17N amino acid replacement) that is tagged at the N-terminal end with mCherry. The coding sequence is also tagged with Tag:AsLOV2, rendering the protein photoactivatable.

(Wang et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsap\RAC1
Tagged with
mCherry
Tag:AsLOV2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Photo-inactivation of Hsap\RAC1PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 at the rear of border cell clusters expressing Hsap\RAC1PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 under the control of Scer\GAL4slbo.2.6 promotes protrusion at the front of the cluster.

      Photo-inactivation of Hsap\RAC1PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 in the forward-directed protrusion of the leading cell in border cell clusters expressing Hsap\RAC1PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 under the control of Scer\GAL4slbo.2.6 results in morphological changes at the back of the cluster.

      (Wang et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Photo-inactivation of Hsap\RAC1PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1 at the front of border cell clusters expressing Hsap\RAC1PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1, EgfrDN.Scer\UAS and PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 results in local retraction.

      (Wang et al., 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Encodes a photoinactivatable form of Hsap\RAC1.

      (Wang et al., 2010)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Hsap\RAC1PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Asat\PT1
      Hsap\RAC1PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1
      Hsap\RAC1PA.T17N.UAS.mCherry,Tag:AsLOV2
      Name Synonyms
      Secondary FlyBase IDs
        References (5)