FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Klp61F3YF.Ubi-p63E.Tag:MYC
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General Information
Symbol
Dmel\Klp61F3YF.Ubi-p63E.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0244462
Feature type
allele
Associated gene
Dmel\Klp61F
Associated Insertion(s)
Carried in Construct
P{Ubi-Myc-Klp61F.3YF}
Key Links
Transgenic product class
missense_variant
(Garcia et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Garcia et al., 2009)
Mutagen
in vitro construct
(Garcia et al., 2009)
Progenitor genotype
Carried in construct
P{Ubi-Myc-Klp61F.3YF}
Cytology
Description

A Ubi-p63E promoter drives expression of a mutated form of Klp61F (containing the amino acid replacements Y23, Y152, and Y207) which is tagged with Tag:MYC.

(Garcia et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
Ubi-p63E
Encoded product / tool
Klp61F
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos derived from Klp61F07012/+ females carrying Klp61F3YF.Ubi-p63E.T:Hsap\MYC show a higher incidence of spindles with multiple microtubule organising centers during cycles 1-9 than seen in control embryos. The mutant embryos also show "promiscuous microtubules", including microtubule spurs and microtubules interacting between neighbouring spindles, during cycles 11-13. These promiscuous microtubules are seen in metaphase (at a similar level as occurs in embryos derived from Klp61F07012/+ females with no transgene) and also in anaphase (at a significantly increased level than occurs in embryos derived from Klp61F07012/+ females with no transgene).

      (Garcia et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Klp61F3YF.Ubi-p63E.Tag:MYC fails to rescue Klp61F07012

      (Garcia et al., 2009)
      Comments

      Klp61F3YF.Ubi-p63E.T:Hsap\MYC does not rescue the pupal lethality of Klp61F07012 homozygotes or the incidence of aneuploidy in Klp61F07012 larval neuroblasts.

      Klp61F3YF.Ubi-p63E.T:Hsap\MYC rescues anastral spindles in embryos derived from heterozygous Klp61F07012 females. However, the monopolar spindle defects are not rescued in these animals.

      (Garcia et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Klp61F3YF.Ubi-p63E.T:Hsap\MYC
      Klp61F3YF.Ubi-p63E.Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (1)