FlyBase Allele Report: Dmel\Vrp1[D30]

General Information

Symbol

Dmel\Vrp1^D30

Species

D. melanogaster

Name

FlyBase ID

FBal0247587

Feature type

allele

Associated gene

Dmel\Vrp1

Associated Insertion(s)

Carried in Construct

Also Known As

WIP^D30, dWIP^D30

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Massarwa et al., 2007)

Progenitor genotype

P{EPgy2}Vrp1^EY02177

(Massarwa et al., 2007)

Caused by aberration

Df(2R)Vrp1^D30

Cytology

Description

Excision of the progenitor insertion, resulting in a deletion that uncovers Vrp1 and five additional proximal transcription units.

(Massarwa et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male sterile (with Vrp1^S1946), with Scer\GAL4^Mef2.PR, Vrp1^UAS.cMa

(Rotkopf et al., 2011)

Phenotype Manifest In

actin filament (with Vrp1^KO)

(Rotkopf et al., 2011)

actin filament (with Vrp1^S1946), with Scer\GAL4^Mef2.PR, Vrp1^UAS.cMa

(Rotkopf et al., 2011)

dorsal acute muscle cell | embryonic stage

(Massarwa et al., 2007)

dorsal pharyngeal muscle cell

(Haralalka et al., 2014)

embryonic myoblast

(Sens et al., 2010)

fusion competent cell

(Sens et al., 2010)

head cyst cell (with Vrp1^KO)

(Rotkopf et al., 2011)

head cyst cell (with Vrp1^S1946), with Scer\GAL4^Mef2.PR, Vrp1^UAS.cMa

(Rotkopf et al., 2011)

muscle founder cell

(Sens et al., 2010)

myoblast | embryonic stage

(Berger et al., 2008, Massarwa et al., 2007)

plasma membrane | embryonic stage

(Berger et al., 2008)

seminal vesicle (with Vrp1^S1946), with Scer\GAL4^Mef2.PR, Vrp1^UAS.cMa

(Rotkopf et al., 2011)

spermatid cyst (with Vrp1^KO)

(Rotkopf et al., 2011)

spermatid cyst (with Vrp1^S1946), with Scer\GAL4^Mef2.PR, Vrp1^UAS.cMa

(Rotkopf et al., 2011)

visceral muscle fiber | embryonic stage

(Massarwa et al., 2007)

Detailed Description

Statement

Reference

Mutant stage 16 embryos show a severe myoblast fusion defect in the dorsal pharyngeal muscle.

(Haralalka et al., 2014)

Longitudinal muscle development is not significantly disturbed in Vrp1^f06715/Vrp1^D30 transheterozygote embryos.

(Rudolf et al., 2014)

Expression of Vrp1^Scer\UAS.cMa under the control of Scer\GAL4^Mef2.PR in a Vrp1^S1946/Vrp1^D30 mutant background produces male sterile flies. The testis contain mature cysts that do not undergo appreciable coiling, cysts do not occupy the base of the testis tube and the seminal vesicle is completely empty of released sperm. The microfilaments and nuclei of late-stage cysts show significant structural alterations. There is a consistent failure to achieve the intertwined and tightly packed organisation of head cyst cell F-actin arrays and associated spermatid nuclei. The microfilament arrays appear sparse and misshapen, and the normally tight and uniformly oriented bundles of nuclei are loosely packed and partially split.

The microfilaments and nuclei of Vrp1^KO/Vrp1^D30 mutant late-stage spermatid cysts show significant structural alterations. There is a consistent failure to achieve the intertwined and tightly packed organisation of head cyst cell F-actin arrays and associated spermatid nuclei. The microfilament arrays appear sparse and misshapen, and the normally tight and uniformly oriented bundles of nuclei are loosely packed and partially split. The robust, transient network of head cyst cell microfilaments seen in wild type is far less frequently observed in Vrp1^KO/Vrp1^D30 mutants (5% of testis compared to 25% in wild type). However, actin-based projections remain properly associated with individual spermatids nuclei at late stages of cyst maturation.

(Rotkopf et al., 2011)

Cytoplasmic material exchange does not occur between the founder cells and fusion competent myoblasts in Vrp1^D30 mutant embryos.

(Sens et al., 2010)

Vrp1^D30 mutant myoblast fusions are arrested during membrane breakdown.

(Berger et al., 2008)

Vrp1^D30 embryos display many individual, unfused myoblasts, which cluster next to mis-positioned muscle fibers exhibiting a thin, abnormal morphology. On average, only 3 DA1 nuclei are observed in each Vrp1^D30 mutant segment.

(Massarwa et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Vrp1^D30 has visceral muscle fiber | embryonic stage phenotype, suppressible by WASp^{UAS.Tag:Myr(Src64B)}/Scer\GAL4^twi.PB

(Massarwa et al., 2007)

Vrp1^D30 has myoblast | embryonic stage phenotype, suppressible by WASp^{UAS.Tag:Myr(Src64B)}/Scer\GAL4^twi.PB

(Massarwa et al., 2007)

Vrp1^D30 has dorsal acute muscle cell | embryonic stage phenotype, suppressible by WASp^{UAS.Tag:Myr(Src64B)}/Scer\GAL4^twi.PB

(Massarwa et al., 2007)

NOT suppressed by

Statement

Reference

Vrp1^D30 has visceral muscle fiber | embryonic stage phenotype, non-suppressible by WASp^UAS.C.GFP/Scer\GAL4^twi.PB

(Massarwa et al., 2007)

Vrp1^D30 has myoblast | embryonic stage phenotype, non-suppressible by WASp^UAS.C.GFP/Scer\GAL4^twi.PB

(Massarwa et al., 2007)

Vrp1^D30 has dorsal acute muscle cell | embryonic stage phenotype, non-suppressible by WASp^UAS.C.GFP/Scer\GAL4^twi.PB

(Massarwa et al., 2007)

Enhancer of

Statement

Reference

Vrp1^D30 is an enhancer of embryonic myoblast phenotype of sns¹⁵

(Kaipa et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The myoblast fusion defects of sns¹⁵ Vrp1^D30 double mutant embryos are more severe than those of sns¹⁵ single mutant embryos.

(Kaipa et al., 2013)

Expression of WASp^{Scer\UAS.C.T:Avic\GFP} under the control of Scer\GAL4^twi.PB fails to suppress the myoblast fusion phenotype found in Vrp1^D30 mutant embryos.

Expression of WASp^{Scer\UAS.T:Myr1} under the control of Scer\GAL4^twi.PB suppresses the myoblast fusion phenotype found in Vrp1^D30 mutant embryos.

(Massarwa et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Vrp1^D30 is rescued by Vrp1^UAS.cMa/Scer\GAL4^twi.PB

(Massarwa et al., 2007)

Not rescued by

Vrp1^D30 is not rescued by Vrp1^ΔC.UAS/Scer\GAL4^twi.PB

(Massarwa et al., 2007)

Comments

Expression of Vrp1^Scer\UAS.cMa exclusively in myotubes under the control of Scer\GAL4^kirre.PR in a Vrp1^D30 mutant background results in significant, although incomplete, rescue.

(Massarwa et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

D-WIP ^D30

(Bulchand et al., 2010)

D-WIP^D30

(Rotkopf et al., 2011)

Vrp1^D30

WIP^D30

(Berger et al., 2008)

Wsp^D30

(Massarwa et al., 2007)

dWIP^D30

(Bothe et al., 2014, Sens et al., 2010)

wip^30D

(Hornbruch-Freitag et al., 2011)

wip^D30

(Hamp et al., 2016, Haralalka et al., 2014, Rudolf et al., 2014, Kaipa et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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