FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\His1RNAi.UAS.cSa
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General Information
Symbol
Dmel\His1RNAi.UAS.cSa
Species
D. melanogaster
Name
FlyBase ID
FBal0248373
Feature type
allele
Associated gene
His1
Associated Insertion(s)
Carried in Construct
P{UAS-His1.RNAi}
Key Links
Transgenic product class
RNAi_reagent
(Siriaco et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Siriaco et al., 2009)
Mutagen
in vitro construct
(Siriaco et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-His1.RNAi}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat corresponding to His1 coding sequences.

(Siriaco et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
His1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of His1dsRNA.Scer\UAS.cSa under the control of Scer\GAL4da.G32 results in lethality at the late larval or early pupal stages.

      Salivary gland polytene chromosomes show a change in structure compared to in wild-type in larvae expressing His1dsRNA.Scer\UAS.cSa under the control of Scer\GAL4da.G32. Most commonly there is a broadening of the chromosome arms without an obvious disruption in the banding pattern. The DNA content of mutant and wild-type chromosomes is similar, but the mutant chromosomes show a 2- to 5-fold increase in their volume. The mutant chromosomes also show dispersion of the heterochromatic chromocenter.

      Expression of His1dsRNA.Scer\UAS.cSa under the control of Scer\GAL4da.G32 results in a reproducible 14bp decrease in nucleosome repeat length from 172 to 158bp in salivary gland chromatin.

      (Siriaco et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      His1RNAi.UAS.cSa
      His1dsRNA.Scer\UAS.cSa
      His1dsRNA.UAS.cSa
      Name Synonyms
      Secondary FlyBase IDs
        References (2)