FlyBase Allele Report: Dmel\E2f1[PIP-3A.UASp.EGFP]

General Information

Symbol

Dmel\E2f1^{PIP-3A.UASp.EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0248937

Feature type

allele

Associated gene

Dmel\E2f1

Associated Insertion(s)

Carried in Construct

P{UASp-GFP-E2f1.PIP-3A}

Key Links

Transgenic product class

missense_variant

(Shibutani et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Shibutani et al., 2008)

Mutagen

in vitro construct

(Shibutani et al., 2008)

Progenitor genotype

Carried in construct

P{UASp-GFP-E2f1.PIP-3A}

Cytology

Description

UASp regulatory sequences drive expression of a mutated form of E2f1 which contains the amino acid replacements I153A, Y156A and Y157A. The protein is also tagged with EGFP.

(Shibutani et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

E2f1

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death | third instar larval stage, with Scer\GAL4^en-e16E

(Davidson and Duronio, 2012)

abnormal cell death | third instar larval stage, with Scer\GAL4^GMR.PU

(Davidson and Duronio, 2012)

abnormal mitotic cell cycle, with Scer\GAL4^en-e16E

(Shibutani et al., 2008)

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^en-e16E

(Davidson and Duronio, 2012)

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^GMR.PU

(Davidson and Duronio, 2012)

decreased cell size, with Scer\GAL4^en-e16E

(Shibutani et al., 2008)

increased cell death, with Scer\GAL4^en-e16E

(Shibutani et al., 2008)

increased occurrence of cell division | embryonic stage, with Scer\GAL4^arm.PS

(Shibutani et al., 2008)

increased occurrence of cell division | third instar larval stage, with Scer\GAL4^arm.PS

(Shibutani et al., 2008)

partially lethal - majority die, with Scer\GAL4^arm.PS

(Shibutani et al., 2008)

partially lethal - majority die, with Scer\GAL4^en-e16E

(Shibutani et al., 2008)

Phenotype Manifest In

Detailed Description

Statement

Reference

Trypsin-dissociated posterior compartment cells from third instar larval wing discs expressing E2f^{PIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E contain a similar proportion of S phase cells to controls. The rate of proliferation in these cells is increased, with ~11% of cells having a G1 DNA content compared to around 28% in controls.

Expression of E2f^{PIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E induces apoptosis in the posterior compartment of intact third instar larval wing discs. A similar increase in apoptosis is seen when the wing disc cells are trypsin-dissociated and quantified using flow cytometry.

An increased number of second mitotic wave cells enter S phase in eye discs expressing E2f^{PIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PU. Increased EdU staining is also seen posterior to the morphogenetic furrow, suggesting an increase in the length of S phase. Unlike in wild type, apoptosis is detected posterior to the morphogenetic furrow and an increase in DNA damage is also seen. The number of cells entering mitosis (assessed by phospho-histone H3 staining) appears unchanged.

Expression of E2f^{PIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E does not induce apoptosis in the G1-arrested epidermal cells of the embryo.

An increased amount of DNA damage is seen in third instar larval wing discs expressing E2f^{PIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E.

(Davidson and Duronio, 2012)

Expression of E2f^{PIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^arm.PS results in ectopic BrdU incorporation in the anterior and posterior midgut in embryos.

Wing discs from larvae expressing E2f^{PIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E in the posterior compartment grow to a normal size and contain both S phase and mitotic cells in the posterior compartment. Extensive apoptosis is seen in the posterior compartment.

Wing disc cells from the posterior compartment which are expressing E2f^{PIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E are smaller than control wing disc cells from the anterior compartment. More of the posterior compartment cells are in S phase and fewer are in G1 compared to wild-type controls.

Adults expressing E2f^{PIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E have grossly abnormal wings.

(Shibutani et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

BacA\p35^UAS.cHa, E2f1^{PIP-3A.UASp.EGFP}, Scer\GAL4^en-e16E has lethal phenotype

(Davidson and Duronio, 2012)

BacA\p35^UAS.cHa, E2f1^{PIP-3A.UASp.EGFP}, Scer\GAL4^en-e16E has abnormal size | third instar larval stage phenotype

(Davidson and Duronio, 2012)

Phenotype Manifest In

Other

Statement

Reference

BacA\p35^UAS.cHa, E2f1^{PIP-3A.UASp.EGFP}, Scer\GAL4^en-e16E has wing disc | posterior | third instar larval stage phenotype

(Davidson and Duronio, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of W⁰⁵⁰¹⁴ partially suppresses the apoptosis seen in the posterior compartment of third instar larval wing discs expressing E2f^{PIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E.

(Davidson and Duronio, 2012)

Xenogenetic Interactions

Statement

Reference

Co-expression of E2f^{PIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP} and BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^en-e16E results in a range of morphological defects in third instar larval wing discs, with many discs exhibiting severe overgrowth in the posterior compartment. In approximately 1/3 of discs the posterior compartment is almost absent.

(Davidson and Duronio, 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

34059

w^*; P{UASp-GFP-E2f1.PIP-3A}10

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

E2f1^{PIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP}

E2f1^{PIP-3A.UASp.EGFP}

E2f^{PIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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