Flies expressing Hsap\TTR^{L55P.Scer\UAS} under the control of Scer\GAL4^GMR.PF exhibit Triton X-100 soluble Hsap\TTR protein 1 day post-eclosion. However, 30 days post-eclosion, no Triton X-100 soluble Hsap\TTR protein is left, with all the protein forming insoluble aggregates. This aggregated Hsap\TTR remains after shutdown of the transgene expression, suggesting its resistance to proteolytic processes.

Increased overexpression of Hsap\TTR^{L55P.Scer\UAS} under the control of two copies of Scer\GAL4^GMR.PF results in wing defects. The wings appear upturned compared with normal wing posture. Males are more affected than females. These flies do not show defects in flight.

Age-matched Hsap\TTR^{L55P.Scer\UAS} overexpression flies show decreased locomotor activity compared to control flies. Although reduced, they still show some nocturnal climbing activity.

Life span is shortened for flies expressing Hsap\TTR^{L55P.Scer\UAS} under the control of two copies of Scer\GAL4^GMR.PF. Hsap\TTR^{L55P.Scer\UAS} flies have a median survival of 22 days in females and 20 days in males (compared to 34 and 32 days respectively in controls).