UAS regulatory sequences drive expression of Ir84a lacking about 200 N-terminal amino acids. The endogenous signal sequence of Ir84a is replaced with Tag:SS(hCALR) and fused to EGFP.
Neurons co-expressing Ir84aΔN.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP and Ir8aScer\UAS.cAa under the control of Scer\GAL4Or22a.7.717 fail to respond to phenylacetaldehyde.