FlyBase Allele Report: Dmel\hfp[NIG.12085R]

General Information

Symbol

Dmel\hfp^NIG.12085R

Species

D. melanogaster

Name

FlyBase ID

FBal0257011

Feature type

allele

Associated gene

Dmel\hfp

Associated Insertion(s)

Carried in Construct

P{NIG.12085R}

Also Known As

UAS-hfp RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Mitchell et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Mitchell et al., 2010)

Mutagen

in vitro construct

(Mitchell et al., 2010)

Progenitor genotype

Carried in construct

P{NIG.12085R}

Cytology

Description

UASt regulatory sequences drive expression of a hfp RNAi construct.

(Mitchell et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-12085R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | somatic clone, with Scer\GAL4^Act5C.PP

(Mitchell et al., 2010)

increased cell growth rate, with Scer\GAL4^Act5C.PP

(Cranna et al., 2011)

Phenotype Manifest In

wing disc | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PP

(Mitchell et al., 2010)

Detailed Description

Statement

Reference

pUf68^NIG.12085R flip-out clones exhibit increased cell death.

(Cranna et al., 2011)

Wing disc clones expressing pUf68^NIG.12085R under the control of Scer\GAL4^Act5C.PP display extensive cell death. Accumulation of cells under the basal lamina in the wing pouch is observed.

(Mitchell et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^Act5C.PP, hfp^NIG.12085R has increased cell death | somatic clone | third instar larval stage phenotype

(Mitchell et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^Act5C.PP, hfp^NIG.12085R has wing disc | somatic clone | third instar larval stage phenotype, suppressible by hay^GD4213, Scer\GAL4^Act5C.PP

(Mitchell et al., 2010)

BacA\p35^UAS.cHa, Scer\GAL4^Act5C.PP, hfp^NIG.12085R has nucleolus | somatic clone | third instar larval stage phenotype, suppressible by hay^GD4213, Scer\GAL4^Act5C.PP

(Mitchell et al., 2010)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^Act5C.PP, hfp^NIG.12085R has wing disc | somatic clone | third instar larval stage phenotype

(Mitchell et al., 2010)

BacA\p35^UAS.cHa, Scer\GAL4^Act5C.PP, hfp^NIG.12085R has nucleolus | somatic clone | third instar larval stage phenotype

(Mitchell et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa in wing disc clones expressing pUf68^NIG.12085R under the control of Scer\GAL4^Act5C.PP results in large rounded cells 72 hours after clone induction. These enlarged cells are removed from the apical surface of the wind disc epithelium and extruded basally, and all mutant cells undergo cell death. Increased nucleolar size is observed in these cells.

Increased nucleolar size observed in cells expressing BacA\p35^Scer\UAS.cHa and pUf68^NIG.12085R under the control of Scer\GAL4^Act5C.PP is suppressed by co-expression of hay^GD4213.

(Mitchell et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

NIG-Fly

12085R-2

P{NIG.12085R}2

12085R-4

P{NIG.12085R}4