FlyBase Allele Report: Dmel\ash1[HMS00582]

General Information

Symbol

Dmel\ash1^HMS00582

Species

D. melanogaster

Name

FlyBase ID

FBal0257268

Feature type

allele

Associated gene

Dmel\ash1

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00582}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00582}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00582

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^{M.UAS.Tag:FLAG}

(Azpurua et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

abnormal size | third instar larval stage, with Scer\GAL4^Cg.PA

(Zhou et al., 2019)

lethal, with Scer\GAL4^Act5C.PI

(Dietz et al., 2015)

lethal, with Scer\GAL4^en.PU

(Dietz et al., 2015)

partially lethal - majority die, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

visible | adult stage, with Scer\GAL4^en.PU

(Dietz et al., 2015)

visible | adult stage, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

visible | adult stage, with Scer\GAL4^nub.PK

(Dietz et al., 2015)

Phenotype Manifest In

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Cg.PA

(Zhou et al., 2019)

eye, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

mitochondrion | third instar larval stage, with Scer\GAL4^Cg.PA

(Zhou et al., 2019)

posterior crossvein, with Scer\GAL4^en.PU

(Dietz et al., 2015)

posterior crossvein, with Scer\GAL4^nub.PK

(Dietz et al., 2015)

wing blade, with Scer\GAL4^nub.PK

(Dietz et al., 2015)

Detailed Description

Statement

Reference

Expressing ash1^HMS00582 under the control of Scer\GAL4^Cg.PA leads to fragmented mitochondria in the third instar larval fat body (as assessed by MitoGFP).

(Zhou et al., 2019)

Expression of ash1^HMS00582 results in partial pupal lethality - the escapers display small deformed eyes (with Scer\GAL4^ey.PH) and blistered wing with ectopic vein formation at posterior crossvein (PCV) at 25[o]C, while no phenotype is observed at 18[o]C (with Scer\GAL4^nub.PK). Expression controlled by Scer\GAL4^Act5C.PI is lethal and pupal lethal with the Scer\GAL4^en.PU driver - at 18[o]C a few escapers can be recovered and these have ectopic vein at PCV.

(Dietz et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

33705

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00582}attP2/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ash1^HMS00582

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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