Expression of Gr64fScer\UAS.cJa in Df(3L)ΔGr64 mutants under the control of Scer\GAL4Gr5a.1 fully restores trehalose sensation in a behavioural assay.
Similarly to wild-type flies, Df(3L)ΔGr64 mutant flies expressing Gr64fScer\UAS.cJa under the control of Scer\GAL4Gr5a.1display high frequencies of action potentials in response to trehalose.
In contrast to wild-type flies, Df(3L)ΔGr64 mutant flies expressing Gr64fScer\UAS.cJa under the control of Scer\GAL4Gr5a.1 do not show a behavioural preference for sucrose over fructose. In contrast to wild-type flies, the mutant flies display only a minimal increase in action potentials in response to sucrose.
Df(3L)ΔGr64 mutants expressing both Gr64fScer\UAS.cJa and Gr64aScer\UAS.cJa under the control of Scer\GAL4Gr5a.1 show a behavioural preference for sucrose over fructose that is very similar to that of wild-type flies. The wild-type frequency of action potentials in response to sucrose is also restored in these animals.
In contrast to wild-type flies, Df(3L)ΔGr64 mutant flies expressing Gr64fScer\UAS.cJa under the control of Scer\GAL4Gr5a.1 display only a minimal increase in action potentials in response to maltose and they do not show a behavioural preference for maltose over fructose.
Df(3L)ΔGr64 mutants expressing both Gr64fScer\UAS.cJa and Gr64aScer\UAS.cJa under the control of Scer\GAL4Gr5a.1 show a behavioural preference for maltose over fructose that is very similar to that of wild-type flies. The wild-type frequency of action potentials in response to maltose is also restored in these animals.
Expression of Gr64fScer\UAS.cJa under the control of Scer\GAL4Gr5a.1 partially restores the preference for glucose over fructose and action potentials in response to glucose in Df(3L)ΔGr64 mutants.
Co-expression of Gr64aScer\UAS.cJa and Gr64fScer\UAS.cJa under the control of Scer\GAL4Gr5a.1 restores normal preference for glucose over fructose and wild-type frequencies of glucose dependent actin potentials.