Knockdown of Appl through expression of Appl^{G3.dsRNA.Scer\UAS} under the control of Scer\GAL4^{Mef2.247.Switch} does not impair response to negative odors (or to accompanying electric shocks).

Flies experiencing knockdown of Appl though expression of Appl^{G3.dsRNA.Scer\UAS} under the control of Scer\GAL4^Mef2.247 or Scer\GAL4^da.Switch.PT respond normally to shocks.

There are no significant differences in memory scores for flies expressing Appl^{G3.dsRNA.Scer\UAS} under the control of Scer\GAL4^{Mef2.247.Switch} compared to controls 2 hours after a single memory condition cycle. Neither learning, short-term memory, nor 2 hour anesthesia-resistant memory are sensitive to Appl^{G3.dsRNA.Scer\UAS} expression. After massed conditioning comprising five consecutive repeated cycles of training, scores at 24 hours are not significantly different when Appl^{G3.dsRNA.Scer\UAS} is expressed until the control of Scer\GAL4^{Mef2.247.Switch}.

Analysis of long-term memory after five spaced conditioning cycles reveals that flies expressing Appl^{G3.dsRNA.Scer\UAS} under the control of Scer\GAL4^{Mef2.247.Switch} display a memory score significantly lower than controls. When the flies are not fed with RU, both mutants and controls exhibit normal performance at 24 hours after spaced conditioning.