FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\HTT18Q.Ex1.UAS.EGFP.Tag:NLS(Unk)
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General Information
Symbol
Hsap\HTT18Q.Ex1.UAS.EGFP.Tag:NLS(Unk)
Species
H. sapiens
Name
FlyBase ID
FBal0258492
Feature type
allele
Associated gene
Hsap\HTT
Associated Insertion(s)
Carried in Construct
P{UAS-nls.Htt.18Q-EGFP}
Key Links
Transgenic product class
inframe_deletion
(Doumanis et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Doumanis et al., 2009)
Mutagen
in vitro construct
(Doumanis et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-nls.Htt.18Q-EGFP}
Cytology
Description

UASt regulatory sequences drive expression of Hsap\HTT exon 1 containing a 18Q expansion, fused to EGFP with a nuclear localization signal at the N-terminus.

(Doumanis et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\HTT
Tagged with
EGFP
Tag:NLS(Unk)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Hsap\HTT18Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 in larval body wall sensory neurons under the control of Scer\GAL4RluA1 has no apparent effect on larval da neuron viability.

      Expression of Hsap\HTT18Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 in larval body wall sensory neurons under the control of Scer\GAL4RluA1 does not result in the formation of cytoplasmic aggregates.

      Expression of Hsap\HTT18Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 in larval body wall sensory neurons under the control of Scer\GAL4RluA1 does not affect dendrite elaboration in da neurons of the dorsal cluster. Dendrites fully cover the field and project towards the dorsal midline to meet the contralateral dendrites, and also fully extend to segment boundaries.

      The speed of locomotion is not different in Hsap\HTT18Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 expressing larva (driven by Scer\GAL4RluA1) compared to wild-type controls. The trajectory of larval locomotion is also not affected.

      Segmental nerve bursting activity is normal in larvae expressing Hsap\HTT18Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 under the control of Scer\GAL4RluA1.

      (Nishimura et al., 2010)

      Flies co-expressing Hsap\HTT18Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT18Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 in the developing eye through the Scer\GAL4GMR.PF driver exhibit a normal eye phenotype.

      (Doumanis et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Overexpression of RhebScer\UAS.cPa in the developing eye of Hsap\HTT18Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 mutants (both transgene under the control of Scer\GAL4GMR.PF results in a rough eye phenotype with bristle disorganization.

      (Doumanis et al., 2009)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Hsap\HTT18Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Uuuu\nls-Unk
      Hsap\HTT18Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls-KKKRKV
      Hsap\HTT18Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1
      Hsap\HTT18Q.Ex1.UAS.EGFP.Tag:NLS(Unk)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)