Expression of Fitm^GD3580 under the control of either Scer\GAL4^αTub84B.PL or Scer\GAL4^Mef2.PR leads to reduced climbing capability and flightlessness in both young and older flies, expression under Scer\GAL4^C7 impairs in older flies only (both climbing and flight ability is comparable to controls at 4 days after eclosion). Expression driven by either Scer\GAL4^elav.PLu or Scer\GAL4^nSyb.PU (both with UAS-Dicer2 to enhance RNAi efficiency) does not have significant effect on the adult climbing or flight ability regardless of age.

Scer\GAL4^αTub84B.PL or Scer\GAL4^elav.PLu-driven expression of Fitm^KK107999 impairs adult hearing: while the threshold for sound-evoked compound action potential is unaffected, the mechanical amplification gain (provided by auditory sensory neuron motility) is reduced and the best frequency of the antennal sound receiver is higher relative to controls.Expression under the Scer\GAL4^C7 driver results in significantly diminished size of lipid droplets in the fat body of both young and older adult flies.

Expression of CG10671^KK107999 under the control of Scer\GAL4^sal.BO (in combination with a Dicer-2 transgene to enhance RNAi efficiency) does not result in larval lethality at the L2/L3 transition.