FlyBase Allele Report: Dmel\RecQ5[D1]

General Information

Symbol

Dmel\RecQ5^D1

Species

D. melanogaster

Name

FlyBase ID

FBal0261080

Feature type

allele

Associated gene

Dmel\RecQ5

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Nakayama et al., 2009)

Progenitor genotype

RecQ5^BG01967

(Nakayama et al., 2009)

Cytology

Description

Imprecise excision of P{GT1}RecQ5^BG01967 generates a deletion in RecQ5 that removes 1641bp of RecQ5 from the 2st exon to the 2nd intron, along with an insertion of 13bp.

(Nakayama et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | maternal effect

(Sakurai et al., 2011, Nakayama et al., 2009)

abnormal mitotic cell cycle | maternal effect (with RecQ5^D2)

(Sakurai et al., 2011)

fertile

(Nakayama et al., 2009)

radiation sensitive (with Df(3L)fz-D21)

(Nakayama et al., 2009)

viable

(Nakayama et al., 2009)

Phenotype Manifest In

centrosome | maternal effect

(Sakurai et al., 2011)

centrosome | maternal effect (with RecQ5^D2)

(Sakurai et al., 2011)

embryo | maternal effect

(Sakurai et al., 2011, Nakayama et al., 2009)

embryo | maternal effect (with RecQ5^D2)

(Sakurai et al., 2011)

nucleus | maternal effect

(Sakurai et al., 2011, Nakayama et al., 2009)

nucleus | maternal effect (with RecQ5^D2)

(Sakurai et al., 2011)

testis | adult stage (with RecQ5^D2)

(Sakurai et al., 2014)

waste bag (with RecQ5^D2)

(Sakurai et al., 2014)

Detailed Description

Statement

Reference

RecQ5^D1/RecQ5^D2 mutant adult males do not show any obvious defects in the meiotic divisions and spermiogenesis, no gross morphological abnormalities are seen in spermatogonia, spermatocytes, spermatids or other cells in the mutant testes and active motile sperm are present. However, the apical region of the mutant testes is swelled compared to controls and the mutant testes display increased frequency of waste bags.

(Sakurai et al., 2014)

Embryos lacking maternally derived RecQ5 (i.e. from RecQ5^D1/RecQ5^D2 homozygous mothers), display asynchronous nuclei. Most comprised of pairs of nuclei, but sometimes more than three irregular nuclei are found to be clustered together. These pairs of abnormal nuclei are clonally generated from single parent nuclei. The parent nucleus and its daughter nuclei proceed synchronously with the surrounding nuclei in prophase, metaphase, anaphase, telophase and interphase.

The frequency of DNA bridging in embryos from RecQ5^D1 mutant mothers is 4-fold higher than that in wild-type embryos at cycles 10-13. The lack of maternal RecQ5 causes defects in chromosome segregation such as un-wreathing of the knot-like structure that forms between sister chromatids.

Spindle microtubule dynamics in RecQ5^D1/RecQ5^D2 embryos are indistinguishable in terms of shape and size from that in wild-type embryos.

(Sakurai et al., 2011)

Homozygous RecQ5^D1 mutants are viable and fertile with no apparent morphological abnormalities.

Embryos derived from RecQ5^D1 homozygous mothers display normal syncytial nuclei division. However, every mutant embryo has at least 1 abnormal nucleus at cycle 13. In addition, the irregular nuclei are round in shape, even at anaphase, whereas the other nuclei show condensed chromosomes. The irregular nuclei exit from the synchronous cycles of normal syncytial mitosis.

Embryos derived from females heterozygous for wild-type and RecQ5^D1 are similar to wild-type embryos.

RecQ5^D1/Df(3L)fz-D21 mutant larval neuroblasts subjected to 2.2 Gy of X-ray irradiation show a 3-fold greater frequency of aberration than wild-type neuroblasts subjected to the same degree of irradiation. The majority of aberrations generated were chromatid or isochromatid breaks.

(Nakayama et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

RecQ5^D1 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible by lok^p6

(Sakurai et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

RecQ5^D1 has embryo | maternal effect phenotype, suppressible by lok^p6

(Sakurai et al., 2011)

RecQ5^D1 has nucleus | maternal effect phenotype, suppressible by lok^p6

(Sakurai et al., 2011)

RecQ5^D1 has centrosome | maternal effect phenotype, suppressible by lok^p6

(Sakurai et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The inactivation of centrosome associated with asynchronous nuclei in RecQ5^D1 embryos is suppressed when maternal lok^p6 is also absent from the embryo. In addition, the nuclear descent from the cortex is also suppressed.

(Sakurai et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

RecQ5^D1 is rescued by RecQ5^UAS.cNb/Scer\GAL4^arm.PS

(Nakayama et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

RecQ5^D1

(Sakurai et al., 2011, Nakayama et al., 2009)

recq5^D1

(Sakurai et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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