Expression of GlyS^HMS01279 under the control of Scer\GAL4^elav.PLu improves the climbing performance of 31-day-old, but not 5-day-old flies, as compared to age-matched controls.

Expression of GlyS^HMS01279 under the control of Scer\GAL4^Mef2.PR is able to significantly reduce the size of autophagosomes in the muscles of chloroquine-treated larvae starved on low-nutrient food for 6 hours. The number of autophagosomes is not significantly changed compared to controls. The myopathy normally seen in starved larvae treated with chloroquine is significantly reduced, with a much-improved sarcomere structure and much less distortion of the intermyofibrillar spaces.

No appreciable effect on autophagosome size is seen when GlyS^HMS01279 is expressed in the fat body under the control of Scer\GAL4^cg.PU.

After 3 or 6 hours of starvation, expression of GlyS^HMS01279 under the control of Scer\GAL4^Mef2.PR significantly improves the crawling defects seen in chloroquine-treated larvae compared to controls. After 6 hours starvation, crawling is negatively affected in the absence of chloroquine treatment. In well-fed larvae there is little difference between crawling time compared to controls, either in the presence or absence of chloroquine.