FB2026_02 , released June 18, 2026
Allele: Dmel\OrcoUAS.GFP
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General Information
Symbol
Dmel\OrcoUAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0263701
Feature type
allele
Associated gene
Dmel\Orco
Associated Insertion(s)
Carried in Construct
P{UAS(-FRT)GFP-Or83b}
Key Links
Transgenic product class
wild_type
(Louis et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Louis et al., 2008)
Mutagen
FLPase
(Louis et al., 2008)
Progenitor genotype
OrcoFRT.UAS.GFP
(Louis et al., 2008)
Carried in construct
P{UAS(-FRT)GFP-Or83b}
Cytology
Description

The FRT cassette has been excised, allowing expression of the Orco coding region which is tagged at the N-terminal end with GFP under the control of UAS regulatory sequences.

(Louis et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Orco
Tagged with
GFP
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of OrcoScer\UAS.T:Avic\GFP in one olfactory sensory neuron, under the control of Scer\GAL4Or42b.PF and in an Orco1/Orco2, reduces the strength of 'weathervaning' in chemotaxis, compared to wild-type larvae with bilateral function. These larvae exhibit the reorientation bais observed in wild-type.

      (Gomez-Marin and Louis, 2014)

      Larvae with olfactory input restricted to a single unilateral Or42a-expressing olfactory receptor neuron (expressing OrcoScer\UAS.T:Avic\GFP under the control of Scer\GAL4Or42a.PF in a single olfactory receptor neuron in a Orco1/Orco2 background) navigate poorly and show tortuous paths in an assay to study the response to a stable gradient of the odour ethyl butyrate, failing to robustly orient towards the gradient.

      (Gomez-Marin et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments

      Orco1/Orco2 larvae expressing OrcoScer\UAS.T:Avic\GFP under the control of Scer\GAL4Or42a.PF unilaterally in single olfactory sensory neuron on one side of the head are able to navigate up an exponential gradient of the odorant isoamyl acetate, although their paths meander more than Orco1/Orco2 larvae expressing OrcoScer\UAS.T:Avic\GFP under the control of Scer\GAL4Or42a.PF bilaterally in both Or42a-expressing olfactory sensory neurons. Unilaterally rescued animals in which the right olfactory sensory neuron is functional perform better than unilaterally rescued animals in which the left olfactory sensory neuron is functional.

      Orco1/Orco2 larvae expressing OrcoScer\UAS.T:Avic\GFP under the control of Scer\GAL4Or1a.PF bilaterally in both Or1a-expressing olfactory sensory neurons detect an exponential methyl benzoate odorant gradient with sufficient accuracy to reach the peak zone of the gradient field in 60% of cases. Orco1/Orco2 larvae expressing OrcoScer\UAS.T:Avic\GFP under the control of Scer\GAL4Or1a.PF unilaterally in single olfactory sensory neuron on one side of the head have a low chemotaxis score in this assay and while larvae in which the right olfactory sensory neuron is functional show some rescue of chemotaxis compared to Orco1/Orco2 larvae, larvae in which the left olfactory sensory neuron is functional show no rescue of chemotactic behaviour.

      (Louis et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      OrcoScer\UAS.T:Avic\GFP
      OrcoUAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (4)