FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\PGRP-LB500.RNAi.UAS
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General Information
Symbol
Dmel\PGRP-LB500.RNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0265814
Feature type
allele
Associated gene
Dmel\PGRP-LB
Associated Insertion(s)
Carried in Construct
P{UAS-PGRP-LB.RNAi.500}
Key Links
Transgenic product class
RNAi_reagent
(Zaidman-Remy et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Zaidman-Remy et al., 2006)
Mutagen
in vitro construct
(Zaidman-Remy et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-PGRP-LB.RNAi.500}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat of the first 500bp of PGRP-LB coding sequence.

(Zaidman-Remy et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
PGRP-LB
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Animals expressing PGRP-LB500.dsRNA.Scer\UAS under the control of Scer\GAL4da.G32 at 29[o]C show significant lethality. Adult escapers raised at 25[o]C are viable and show no visible difference to wild-type flies.

      Animals expressing PGRP-LB500.dsRNA.Scer\UAS under the control of Scer\GAL4da.G32 exhibit reduced digestion of a "["[14]C"]"DAP-radio-labelled TCT dimer in both unchallenged and bacteria-challenged flies, indicating a significant decrease in hemolymph amidase activity.

      Inactivation of PGRP-LB through expression of PGRP-LB500.dsRNA.Scer\UAS (under the control of Scer\GAL4da.G32) does not induce increased susceptibility to infection, as determined from fly survival rates following injection of E.carotovora.

      (Zaidman-Remy et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      PGRP-LB500.RNAi.UAS
      PGRP-LB500.dsRNA.Scer\UAS
      PGRP-LB500.dsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)