UAS regulatory sequences drive expression of a mutant form of Ubx in which the PDWL amino acid motif starting at position 277 has been mutated to alanines and the entire C-terminal has been deleted. The construct is tagged with a Tag:HA epitope.
homeotic, with Scer\GAL4arm.PS
embryonic abdominal segment 1 | ectopic, with Scer\GAL4arm.PS
embryonic thoracic segment, with Scer\GAL4arm.PS
Expressing UbxW1A-CTΔ.Scer\UAS.T:Ivir\HA1 ubiquitously under the control of Scer\GAL4arm.PS causes homeotic transformations to A1 in segments anterior to the wild type A2 segment, as assessed by denticle pattern.