UAS regulatory sequences drive expression of a mutant form of Ubx that lacks the N-terminal domain up to amino acid 282, and in which the EKQAQAQK amino acid motif at position 350 has been mutated to AAVAVAVA. The construct is tagged with a Tag:HA epitope.
Expressing UbxNTΔ-QA.Scer\UAS.T:Ivir\HA1 ubiquitously under the control of Scer\GAL4arm.PS causes weak homeotic transformations to A1 in segments anterior to the wild type A2 segment, as assessed by denticle pattern.