UAS regulatory sequences drive expression of a dimerization-deficient version of a coiled-coil (CC) motif from yeast GCN4 fused to the N terminus of fu. The construct is tagged with a Tag:HA epitope.
Expression of fuCCm.Scer\UAS.T:Ivir\HA1 in the male wing under the control of Scer\GAL4Bx-MS1096 does not produce any anterior overgrowth.