Flies expressing Pgam5^NIG.14816R under the control of Scer\GAL4^da.G32 are sensitive to heat shock stress and die sooner than control flies. Heat shock sensitivity is also seen when Pgam5^NIG.14816R is expressed under the control of Scer\GAL4^hs.PU for 4X 30 minutes at 37[o]C prior to heat shock treatment.

Expression of Pgam5^NIG.14816R in the fat body (under the control of Scer\GAL4^yolk), hemocytes (Scer\GAL4^Pxn.PS), eye (Scer\GAL4^hs.2sev), bristle (Scer\GAL4^sca-P309) or mediodorsal parts of the thoracic and abdominal segments (Scer\GAL4^pnr-MD237) has no effect on heat shock sensitivity compared to controls. An increase in sensitivity is seen when Pgam5^NIG.14816R is expressed in the mushroom body under the control of either Scer\GAL4^c739, Scer\GAL4^ey-OK107 or Scer\GAL4^Tab2-201Y and expression under the control of Scer\GAL4^elav-C155 also induced the vulnerability of flies to heat shock. No change in sensitivity is seen when Pgam5^NIG.14816R is expressed in dopaminergic neurons under the control of Scer\GAL4^ple.PF.