FlyBase Allele Report: Dmel\Mtpα[KO]

General Information

Symbol

Dmel\Mtpα^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0267653

Feature type

allele

Associated gene

Dmel\Mtpα

Associated Insertion(s)

TI{TI}Mtpα^KO

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

Mutagen

ends-out gene targeting

(Kishita et al., 2012)

Progenitor genotype

Associated Insertion(s)

TI{TI}Mtpα^KO

Cytology

Description

Sequence encoding the 3-enoyl-CoA hydratase domain of Mtpα has been replaced by a w^+* marker.

(Kishita et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2L:9,574,839..9,576,661 [-]

Comment:

Part of Mtpα has been replaced by a w^+* marker. The position of the deletion endpoints on the reference sequence was inferred from the reported primer sequences used to generate the homology arms.

(Kishita et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of steatotic liver disease

CEA

(Kishita et al., 2012)

model of lipid metabolism disorder

CEA

(Kishita et al., 2012)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | larval stage

(Tiwari et al., 2020)

abnormal locomotor behavior | adult stage

(Kishita et al., 2012)

abnormal starvation stress response | adult stage

(Kishita et al., 2012)

fertile

(Kishita et al., 2012)

short lived

(Kishita et al., 2012)

viable

(Kishita et al., 2012)

Phenotype Manifest In

adult fat body

(Kishita et al., 2012)

lipid droplet

(Kishita et al., 2012)

primary lobe of embryonic/larval lymph gland | larval stage

(Tiwari et al., 2020)

Detailed Description

Statement

Reference

Mtpα^KO homozygous larvae exhibit a large progenitor pool at the expense of differentiated cells in the lymph gland primary lobe.

(Tiwari et al., 2020)

The mean lifespan of mutant male flies (37.6 +/- 0.5 days) is significantly shorter than that of control males (49.6 +/- 0.5 days).

Mutant adults show significantly slower climbing ability than control flies at 3, 10 and 20 days after eclosion.

Mutant females lay approximately 40% fewer eggs compared to wild type.

Mutant adults are susceptible to starvation, the mean survival period under starvation conditions in 29.5 +/- 1.6 hours (compared to 44.6 +/- 1.0 hours in wild-type controls).

Fat body cells of fed mutant flies contain larger lipid droplets compared to cells from wild-type control flies and the mutant flies have higher overall triacylglyceride levels than wild type. The size of the lipid droplets, and the overall triacylglyceride levels are not reduced by starvation in the mutant flies, in contrast to wild type.

(Kishita et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Mtpα^KO

(Tiwari et al., 2020, Kishita et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close