FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Syt1I420E.UAS
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General Information
Symbol
Dmel\Syt1I420E.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0267943
Feature type
allele
Associated gene
Dmel\Syt1
Associated Insertion(s)
Carried in Construct
P{UAS-Syt1.I420E}
Key Links
Transgenic product class
missense_variant
(Paddock et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Paddock et al., 2011)
Mutagen
in vitro construct
(Paddock et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-Syt1.I420E}
Cytology
Description

Amino acid replacement: I420E.

(Paddock et al., 2011)

UAS regulatory sequences drive expression of mutated Syt1 coding sequences.

(Paddock et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Syt1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Syt1I420E.Scer\UAS under the control of Scer\GAL4elav-C155 in a Syt1AD4 mutant background results in embryonic lethality. The embryos complete embryogenesis (through stage 17) but fail to hatch. When manually removed from the egg case, the embryos are virtually immobile. The are microscopic movements within the body wall musculature, but these generally are insufficient to displace the embryo and all visible movement stops within hours. Ultrastructural examination of the synaptic neuropil reveals abundant small clear vesicles. The overall population of synaptic vesicles in the neuropil is correctly localized to synaptic regions, indicating that this mutation does not disrupt the formation or targeting of synaptic vesicles.

      Expression of Syt1I420E.Scer\UAS under the control of Scer\GAL4elav-C155 results in a drop in the amplitude of evoked transmitter relase, in a Syt1AD4 background, compared to expression of Syt1Scer\UAS.cPa.

      Expression of Syt1I420E.Scer\UAS under the control of Scer\GAL4elav-C155 in a wild-type background results in embryonic lethality.

      (Paddock et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Syt1I420E.Scer\UAS
      Syt1I420E.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)