FlyBase Allele Report: Dmel\gbb[UAS.mNSmS1]

General Information

Symbol

Dmel\gbb^UAS.mNSmS1

Species

D. melanogaster

Name

FlyBase ID

FBal0268089

Feature type

allele

Associated gene

Dmel\gbb

Associated Insertion(s)

Carried in Construct

PBac{UAS-gbb.mNSmS1}

Key Links

Transgenic product class

missense_variant

(Akiyama et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Akiyama et al., 2012)

Mutagen

in vitro construct

(Akiyama et al., 2012)

Progenitor genotype

Carried in construct

PBac{UAS-gbb.mNSmS1}

Cytology

Description

UASt regulatory sequences drive expression of a gbb coding sequence containing mutations in the NS and S1 furin cleavage consensus sites.

(Akiyama et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

gbb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing, with Scer\GAL4^ap-md544

(Akiyama et al., 2012)

wing, with Scer\GAL4^ci.PU

(Akiyama et al., 2012)

wing, with Scer\GAL4^dpp.blk1

(Akiyama et al., 2012)

wing, with Scer\GAL4^hh.PU

(Akiyama et al., 2012)

wing disc, with Scer\GAL4^ap-md544

(Akiyama et al., 2012)

wing vein, with Scer\GAL4^ap-md544

(Akiyama et al., 2012)

wing vein, with Scer\GAL4^ci.PU

(Akiyama et al., 2012)

wing vein, with Scer\GAL4^dpp.blk1

(Akiyama et al., 2012)

wing vein, with Scer\GAL4^hh.PU

(Akiyama et al., 2012)

Detailed Description

Statement

Reference

Expression of gbb^{Scer\UAS.mNSmS1} under the control of Scer\GAL4^ap-md544 results in 100% viability and an overall reduction in the size of the wing disc. Expression under the control of Scer\GAL4^hh.PU results in 94% viability.

Expression of gbb^{Scer\UAS.mNSmS1} under the control of Scer\GAL4^ci.PU results in 3% viability, while expression under the control of Scer\GAL4^dpp.blk1 results in 100% lethality. Scer\GAL4^dpp.blk1-driven expression of gbb^{Scer\UAS.mNSmS1} results in pharate adults, when dissected out of their pupal cases, showing macrochaetae and leg defects consistent with a reduction in BMP signaling. Wings from adult survivors show blistered and ectopic venation phenotypes.

(Akiyama et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

gbb^{Scer\UAS.mNSmS1}

gbb^UAS.mNSmS1

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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