FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Nsf2lotus-312
Open Close
General Information
Symbol
Dmel\Nsf2lotus-312
Species
D. melanogaster
Name
FlyBase ID
FBal0268654
Feature type
allele
Associated gene
Dmel\Nsf2
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Ghabrial et al., 2011)
Progenitor genotype
Cytology
Description

Amino acid replacement: I84K.

(Song et al., 2013)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:13,837,562..13,837,562 [-]
Nucleotide change:

T13837562A

Reported nucleotide change:

T?A

Amino acid change:

I84K | Nsf2-PA; I84K | Nsf2-PB; I84K | Nsf2-PC

Reported amino acid change:

I84K

(Song et al., 2013)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Nsf2lotus-312 mutants exhibit tracheal morphogenesis defects. In Nsf2lotus-312 mutant clones the terminal cells are smaller with fewer branches and exhibit a gas-filling gap within the transition zone.

      (Song et al., 2013)

      lotus312 mutants display tracheal terminal cells that are pruned and sometimes fragmented (as if degenerating). Other tracheal cells are smaller than in wild-type. The terminal cell seamless-auto-cellular tube connection is defective. The terminal cells are rounded and contribute minimally to the lumen.

      lotus312 mutant eye clones in a EGUF/hid eye assay (where forced expression of a cell death gene in the eye imaginal disc eliminates wild-type cells, leaving the adult eyes to develop almost entirely from clones of homozygous mutant cells), form eyes of normal size and shape.

      (Ghabrial et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      312
      (Ghabrial et al., 2011)
      Nsf2lotus-312
      lotus312
      (Song et al., 2013)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)