1 day old flies containing large clones of homozygous tissue in the eye have an electroretinogram (ERG) with a significantly reduced amplitude compared to wild type. The ERG amplitude decreases as the flies age.
Aats-metHV/Aats-metFB adult escapers have normal ERGs at 1 day old, while the ERG is severely abnormal in 3 week old flies. The rhabdomeres and overall structure of the photoreceptors appears normal, with the correct number of photoreceptors per ommatidium in 1 day old flies. The total mitochondrial area in the mutant photoreceptors is greater than normal in 1 day and 1 week old flies. Lipid droplets are present in the pigment cells in mutant flies, a phenotype not seen in wild type. The photoreceptors and glial cells progressively degenerate, such that by 3 weeks of age, most photoreceptors are severely affected and many organelles are barely recognisable.
Homozygous and Aats-metHV/Aats-metFB flies have shorter lifespans than normal and are unable to fly.
The myofibrils of the indirect flight muscles appear intact in 1 day old Aats-metHV/Aats-metFB flies, but the mitochondria are clearly abnormal, being larger than normal. The myofibrils show defects in 1 week old flies and the mitochondria are very large.
Aats-metHV/Df(3R)Exel7321 animals die as late third instar larvae or small pupae, and have small imaginal discs and larval brains.