FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\14-3-3εRNAi.UAS.cTa
Open Close
General Information
Symbol
Dmel\14-3-3εRNAi.UAS.cTa
Species
D. melanogaster
Name
FlyBase ID
FBal0270081
Feature type
allele
Associated gene
Dmel\14-3-3ε
Associated Insertion(s)
Carried in Construct
P{UAS-14-3-3ε.RNAi.T}
Key Links
Transgenic product class
RNAi_reagent
(Tsigkari et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Tsigkari et al., 2012)
Mutagen
in vitro construct
(Tsigkari et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-14-3-3ε.RNAi.T}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat of 14-3-3ε sequence, which consists of a 990bp XbaI fragment containing the complete cDNA followed by a 400bp fragment containing the 5' portion of the gene, in the opposite orientation.

(Tsigkari et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
14-3-3ε
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of 14-3-3εdsRNA.Scer\UAS.cTa under the control of Scer\GAL4αTub84B.PL results in a significant reduction in the number of pole cells per gonad in stage 12-13 embryos.

      Expression of 14-3-3εdsRNA.Scer\UAS.cTa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 results in a small but significant reduction in the number of pole cells per gonad in stage 12-13 embryos.

      Expression of 14-3-3εdsRNA.Scer\UAS.cTa under the control of Scer\GAL4how-24B has no significant effect on the number of pole cells per gonad in stage 12-13 embryos.

      (Tsigkari et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      14-3-3εRNAi.UAS.cTa
      14-3-3εdsRNA.Scer\UAS.cTa
      14-3-3εdsRNA.UAS.cTa
      Name Synonyms
      Secondary FlyBase IDs
        References (3)