Expression of Hsap\UTRN^{Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^c355 results in F-actin defects during S9. No abnormalities are seen in the nurse cells, but a delay in border cell migration is often observed. The F-actin bundles that form during S10B appear normal.

Weak expression of Hsap\UTRN^{Scer\UAS.P\T.T:Avic\GFP-EGFP} during S9 under the control of Scer\GAL4^{osk.T:Hsim\VP16} results in no obvious F-actin defects in the nurse cells and border cell migration appears normal. Some disorganisation is seen in the actin bundles that form at stage 10B.

When Hsap\UTRN^{Scer\UAS.P\T.T:Avic\GFP-EGFP} is expressed strongly in the germline of S9 follicles, severe actin defects are observed. In particular, clumps of ring canals and multi-nucleate nurse cells are observed. These defects are not due to incomplete cytokinesis but are the result of nurse cell cortical actin breakdown, as the cortical actin is intact in the earlier stages of oogenesis. Early actin filaments and aggregates are also seen in the posterior nurse cells adjacent to the oocyte at a much higher frequency and severity than observed in control follicles. F-actin "threads' are observed within the nurse cell nuclei and germinal vesicle from stage 6 to stage 9, and occasionally into stage 10A to 10B. Severe defects, including cortical breakdown, multinucleate nurse cells and elongated and disorganised actin bundles, are also seen in the F-actin bundles that form during S10B.