FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Gem2NIG.10419R
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General Information
Symbol
Dmel\Gem2NIG.10419R
Species
D. melanogaster
Name
FlyBase ID
FBal0270461
Feature type
allele
Associated gene
Dmel\Gem2
Associated Insertion(s)
Carried in Construct
P{NIG.10419R}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Japanese National Institute of Genetics, 2012.5.21)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Japanese National Institute of Genetics, 2012.5.21)
Mutagen
in vitro construct
(Japanese National Institute of Genetics, 2012.5.21)
Progenitor genotype
Carried in construct
P{NIG.10419R}
Cytology
Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-10419R
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Gem2NIG.10419R (1 or 2 copies) driven by Scer\GAL4Mef2.PR does not significantly impair flight ability.

      (Borg et al., 2015)

      Expression of Gem2NIG.10419R under the control of Scer\GAL4da.PU causes a drastic reduction in adult viability at 25[o]C or complete lethality at 29[o]C. A marked reduction in lethality can also be observed when Gem2NIG.10419R is expressed in the muscle lineages under the control of Scer\GAL4how-24B or Scer\GAL4c179.

      Flies expressing Gem2NIG.10419R (co-expressed with Dcr-2Scer\UAS.cDa) under the control of Scer\GAL4Mef2.PR show an age-dependent reduction in climbing ability compared to controls. Flies exhibit a progressive age-dependent decline in flight performance and are short-lived compared to controls.

      (Borg and Cauchi, 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Statement
      Reference

      Gem2NIG.10419R, Scer\GAL4da.PU has lethal | heat sensitive phenotype, non-suppressible by Gem3ΔC.UAS, Scer\GAL4da.PU

      (Borg and Cauchi, 2013)
      Other
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Gem3ΔN.Scer\UAS and one copy of Gem2NIG.10419R driven by Scer\GAL4Mef2.PR results in flight defects and partial lethality; co-expression of Gem3ΔN.Scer\UAS and two copies of Gem2NIG.10419R driven by Scer\GAL4Mef2.PR results in lethality.

      (Borg et al., 2015)

      Expression of Gem3ΔC.Scer\UAS fails to suppress the lethality seen when Gem2NIG.10419R is expressed under the control of Scer\GAL4da.PU.

      (Borg and Cauchi, 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Gem2NIG.10419R is rescued by Scer\GAL4da.PU/Gem2UAS.cBa

      (Borg and Cauchi, 2013)
      Comments

      Expression of Gem2Scer\UAS.cBa suppresses the lethality seen when Gem2NIG.10419R is expressed under the control of Scer\GAL4da.PU.

      (Borg and Cauchi, 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Gem2NIG.10419R
      Name Synonyms
      Secondary FlyBase IDs
        References (4)