FlyBase curator comment: this entry is used to capture phenotypic information when the particular allele (or allele combination) used by the author could not be determined but the context of the experiment suggests that the phenotype being described is some kind of loss of function.
98% of embryos lacking both maternal and zygotic wun2 function have wild-type trachea.
79% of embryos lacking both maternal and zygotic wun and wun2 function (derived from females carrying homozygous double mutant wun49 wun2EP2650ex34 germline clones mated to wun wun2 double mutant males) have aberrant tracheal morphology. Breaks in the dorsal trunk plus bulbous chitin accumulation throughout the dorsal and lateral trunk and transverse connectives are seen.
Expression of either wunScer\UAS.T:Avic\GFP-EGFP, wun2Scer\UAS.T:Hsap\MYC or wun2Scer\UAS.T:Avic\GFP under the control of Scer\GAL4btl.PS completely rescues the tracheal defects that are seen in embryos lacking both maternal and zygotic wun and wun2 function.
Expression of wun2H326K.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4btl.PS fails to rescue the tracheal defects that are seen in embryos lacking both maternal and zygotic wun and wun2 function.
Expression of wun2Scer\UAS.T:Hsap\MYC under the control of either Scer\GAL4how-24B or Scer\GAL4drm.7.1 fails to rescue the tracheal defects that are seen in embryos lacking both maternal and zygotic wun and wun2 function.
Embryos lacking both maternal and zygotic wun and wun2 function show defects in paracellular barrier function in the trachea and blood-brain barrier function in the central nervous system in a dextran injection assay.
Expression of either Mmus\Ppap2cScer\UAS.T:Avic\GFP or Mmus\Ppap2bScer\UAS.T:Avic\GFP under the control of Scer\GAL4btl.PS rescues the tracheal defects that are seen in embryos lacking both maternal and zygotic wun and wun2 function.
