FlyBase Allele Report: Dmel\CRMP[supK1]

General Information

Symbol

Dmel\CRMP^supK1

Species

D. melanogaster

Name

FlyBase ID

FBal0277232

Feature type

allele

Associated gene

Dmel\CRMP

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

hexamethylphosphoramide

Nature of the Allele

Allele class

Mutagen

hexamethylphosphoramide

(Morris et al., 2012)

Progenitor genotype

Cytology

Description

Deletion of 153 bp of exon E4-intron I4 sequences of CRMP with an insertion of six nucleotides (Genbank GU452677). The predicted open reading frame created by the E4-I4 fusion terminates at an in-frame nonsense codon within intron I4, probably resulting in a severely truncated product consisting of only the N-terminal 10% of the CRMP protein.

(Morris et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:5,574,702..5,574,854 [+]

Comment:

5381 bp deletion associated with the imprecise excision of P{EP}CRMP^EP3238, which removes the 3' end of exon 4 and the first nucleotide of intron 4 of CRMP.

(Morris et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon collateral | adult stage

(Zhao et al., 2021)

Detailed Description

Statement

Reference

CRMP^supK1 adults show circadian arrhythmicity. Their l-LNvs show a split POT.

(Zhao et al., 2021)

Homozygous, hemizygous, or heteroallelic CRMP^supK1 mutants are viable, fertile, and morphologically normal when grown under standard laboratory conditions.

When tested for short term memory performance 2 minutes after a single aversive Pavlovian training session, CRMP^supK1/CRMP^supIa1 as well as homozygous CRMP^supK1 mutants exhibit significantly reduced performance compared with wild-type control flies. Medium-term memory performance measured 3 hours after a single aversive Pavlovian training session is also reduced in homozygous mutants and heteroallelic mutant animals.

Homozygous CRMP^supK1 mutants exhibit significantly reduced long-term memory performance compared with control animals.

Under free-running conditions (constant darkness), wild-type and CRMP^supK1 mutant animals exhibit similar overall activity. Period length is similar for all tested animals, indicating that the length of the circadian clock is not affected by CRMP^supK1. Compared with wild-type and heterozygous animals, significantly reduced arrhythmic power values indicating the relative strength of rhythmicity of free-running behaviours are observed among CRMP^supK1 homozygotes, CRMP^supK1/Df(3R)noi-B hemizygotes and CRMP^supK1/CRMP^supIa1 heteroallelic animals. Some individual CRMP^supK1 homozygotes and CRMP^supK1/Df(3R)noi-B hemizygotes lack significantly rhythmic behaviours (arrhythmic).

(Morris et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

CRMP^supK1 is an enhancer of visible phenotype of Rac2^EP3118, Scer\GAL4^GMR.PF

(Morris et al., 2012)

NOT Enhancer of

Statement

Reference

CRMP^supK1 is a non-enhancer of visible phenotype of Rac1^N17.UAS, Scer\GAL4^sev.EP

(Morris et al., 2012)

Suppressor of

Statement

Reference

CRMP^supK1 is a suppressor | partially of lethal | pharate adult stage phenotype of Scer\GAL4^GMR.PF, sgg^UAS.cBa

(Morris et al., 2012)

CRMP^supK1 is a suppressor | partially of lethal | pharate adult stage phenotype of Scer\GAL4^ey.PH, sgg^UAS.cBa

(Morris et al., 2012)

CRMP^supK1 is a suppressor | partially of visible | pharate adult stage phenotype of Scer\GAL4^GMR.PF, sgg^UAS.cBa

(Morris et al., 2012)

CRMP^supK1 is a suppressor | partially of visible | pharate adult stage phenotype of Scer\GAL4^ey.PH, sgg^UAS.cBa

(Morris et al., 2012)

CRMP^supK1 is a suppressor | partially of lethal | pupal stage phenotype of Scer\GAL4^GMR.PF, sgg^UAS.cBa

(Morris et al., 2012)

CRMP^supK1 is a suppressor | partially of lethal | pupal stage phenotype of Scer\GAL4^ey.PH, sgg^UAS.cBa

(Morris et al., 2012)

NOT Suppressor of

Statement

Reference

CRMP^supK1 is a non-suppressor of visible phenotype of Rac1^N17.UAS, Scer\GAL4^sev.EP

(Morris et al., 2012)

Other

Statement

Reference

CRMP^supK1, Rac2^EP3118, Scer\GAL4^ey.PH has visible phenotype

(Morris et al., 2012)

CRMP^supK1, Rac1^V12.UAS, Scer\GAL4^sev.EP has visible phenotype

(Morris et al., 2012)

CRMP^supK1, Rac2^EP3118, Scer\GAL4^elav-C155 has visible phenotype

(Morris et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

CRMP^supK1 is an enhancer of ommatidium phenotype of Rac2^EP3118, Scer\GAL4^GMR.PF

(Morris et al., 2012)

NOT Enhancer of

Statement

Reference

CRMP^supK1 is a non-enhancer of eye phenotype of Rac1^N17.UAS, Scer\GAL4^sev.EP

(Morris et al., 2012)

Suppressor of

Statement

Reference

CRMP^supK1 is a suppressor | partially of eye | pharate adult stage phenotype of Scer\GAL4^GMR.PF, sgg^UAS.cBa

(Morris et al., 2012)

CRMP^supK1 is a suppressor | partially of eye | pharate adult stage phenotype of Scer\GAL4^ey.PH, sgg^UAS.cBa

(Morris et al., 2012)

CRMP^supK1 is a suppressor | partially of head | pharate adult stage phenotype of Scer\GAL4^GMR.PF, sgg^UAS.cBa

(Morris et al., 2012)

CRMP^supK1 is a suppressor | partially of head | pharate adult stage phenotype of Scer\GAL4^ey.PH, sgg^UAS.cBa

(Morris et al., 2012)

NOT Suppressor of

Statement

Reference

CRMP^supK1 is a non-suppressor of eye phenotype of Rac1^N17.UAS, Scer\GAL4^sev.EP

(Morris et al., 2012)

Other

Statement

Reference

CRMP^supK1, Rac2^EP3118, Scer\GAL4^ey.PH has eye phenotype

(Morris et al., 2012)

CRMP^supK1, Rac1^V12.UAS, Scer\GAL4^sev.EP has eye phenotype

(Morris et al., 2012)

CRMP^supK1, Rac2^EP3118, Scer\GAL4^elav-C155 has eye phenotype

(Morris et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype resulting from the expression of Ras85D^Scer\UAS.cKa via Scer\GAL4^GMR.PF is suppressed by homozygous CRMP^supK1.

CRMP^supK1 homozygotes expressing sgg^Scer\UAS.cBa under the control of either Scer\GAL4^GMR.PF or Scer\GAL4^ey.PH regularly survive to the pharate adult stage and frequently eclose as adults displaying reduced rough eyes.

Severely reduced eyes are produced in homozygous CRMP^supK1 animals also expressing Rac1^V12.Scer\UAS under the control of Scer\GAL4^sev.EP.

Animals expressing dominant-negative Rac1^N17.Scer\UAS under the control of Scer\GAL4^sev.EP display no discernible differences in the presence or absence of homozygous CRMP^supK1.

The eye phenotype resulting from the expression of Rac2^EP3118 under the control of Scer\GAL4^GMR.PF is enhanced in CRMP^supK1 animals that have reduced eyes with extensively fused ommatidia.

The Scer\GAL4^ey.PH driver produces irregular ommatidial arrangements and reduced eyes in Rac2^EP3118 animals that are homozygous for CRMP^supK1.

Pan-neural expression of Rac2^EP3118 with Scer\GAL4^elav-C155 in CRMP^supK1 homozygotes results in rough eyes.

(Morris et al., 2012)

CRMP^{T:Avic\GFP-EGFP} supports the suppression of the body colour phenotype of b^unspecified, CRMP^supK1 double mutants by r^Su(b).cSa resulting in a brownish cuticle.

CRMP^{fs9a.T:Avic\GFP-EGFP} fails to support the suppression of the body colour phenotype of b^unspecified, CRMP^supA4/CRMP^supK1 double mutants by r^Su(b).cSa resulting in a dark gray cuticle.

CRMP^{fs9b.T:Avic\GFP-EGFP} supports the suppression of the body colour phenotype of b^unspecified, CRMP^supA4/CRMP^supK1 double mutants by r^Su(b).cSa resulting in a brownish cuticle.

(Morris et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

40954

w^*; Adc^b-1; CRMP^supK1

40958

w^*; Adc^b-1; P{PYDGFPfs9a}1a CRMP^supK1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CRMP^supK1

(Titus et al., 2023, Zhao et al., 2021, Morris et al., 2012, Morris et al., 2012)

crmp^supK1

(Jauffred et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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