FlyBase Allele Report: Dmel\Phf7[ΔN2]

General Information

Symbol

Dmel\Phf7^ΔN2

Species

D. melanogaster

Name

FlyBase ID

FBal0277257

Feature type

allele

Associated gene

Dmel\Phf7

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Yang et al., 2012)

Progenitor genotype

P{EPgy2}Phf7^EY03023

(Yang et al., 2012)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion that removes approximately 2kb upstream of the annotated transcription start site and the first 595bp of the transcript. This results in the deletion of amino acid residues 1-137.

(Yang et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates brain cancer

modeled by l(3)mbt^ts1

(Molnar et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased fecundity, with Scer\GAL4^{VP16.nanos.UTR}

(Yang et al., 2017)

decreased fecundity | male

(Yang et al., 2012)

female fertile (with Df(1)Phf7^Δ88)

(Yang et al., 2012)

viable

(Yang et al., 2012)

Phenotype Manifest In

male germline stem cell

(Yang et al., 2012)

male germline stem cell | germline clone

(Yang et al., 2012)

spermatocyte | germline clone

(Yang et al., 2012)

spermatocyte cell spectrosome

(Yang et al., 2012)

spermatogonial cyst | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Yang et al., 2017)

Detailed Description

Statement

Reference

Phf7^ΔN2 homozygous males present significant decreases in fecundity and in the number of Bam-positive spermatogonial cysts, but not in the number of cells per cyst, as compared to controls.

(Yang et al., 2017)

Phf7^ΔN2/Df(1)Phf7^Δ88 females retain full fecundity, but Phf7^ΔN2/Y males show substantially reduced fecundity compared to controls.

The number of germline stem cells (GSCs) per testis is moderately elevated in Phf7^ΔN2/Y males compared to rescued controls. The centrosomes of the mutant GSCs are not as reliably oriented toward the hub-GSC interface as in wild type, although this does not result in an increase in misoriented GSC divisions. The spectrosomes are more likely to be localised to the hub-GSC interface in the mutant testes compared to wild type. The number of spermatogonia generated per GSC is lower than normal.

The number of mutant GSC clones per testis declines steadily over time in animals containing loss of function clones in a rescued background, in contrast to control clones. The number of mutant spermatocyte clones is substantially lower than that of controls in these animals.

(Yang et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Phf7^ΔN2, Scer\GAL4^{VP16.nanos.UTR} has decreased fecundity phenotype, suppressible by Reepl1^CC4, Scer\GAL4^{VP16.nanos.UTR}

(Yang et al., 2017)

Phf7^ΔN2 has decreased fecundity | male phenotype, suppressible by Hsap\PHF7^UASp.cYa

(Yang et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Phf7^ΔN2, Scer\GAL4^{VP16.nanos.UTR} has spermatogonial cyst | adult stage phenotype, suppressible by Reepl1^CC4, Scer\GAL4^{VP16.nanos.UTR}

(Yang et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased fecundity and the decreased number of spermatogonial cysts observed in Phf7^ΔN2 homozygous males are suppressed by additional homozygosity for Reepl1^CC4.

(Yang et al., 2017)

Phf7^ΔN2 significantly rescues germ cell number and organisation in XY animals expressing tra^F.U2af50.

(Yang et al., 2012)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\PHF7^{Scer\UAS.P\T.cYa} rescues the reduced fecundity of Phf7^ΔN2 males.

(Yang et al., 2012)

Complementation and Rescue Data

Rescued by

Phf7^ΔN2 is rescued by Scer\GAL4^{VP16.nanos.UTR}/Phf7^UASp.cYa

(Yang et al., 2017)

Phf7^ΔN2 is rescued by Phf7^3xC.Tag:HA

(Yang et al., 2012)

Phf7^ΔN2 is rescued by Phf7^{Tag:FLAG,Tag:StrepII,Tag:polyHis}

(Yang et al., 2012)

Phf7^ΔN2 is rescued by Phf7^UASp.cYa

(Yang et al., 2012)

Comments

The fecundity defects of Phf7^ΔN2 homozygous males are rescued by the expression of Phf7^{Scer\UAS.P\T.cYa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Yang et al., 2017)

Basal expression of Phf7^{Scer\UAS.P\T.cYa} (in the absence of a Scer\GAL4 driver) rescues the reduced fecundity of Phf7^ΔN2 males.

(Yang et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Phf7^ΔN2

(Bhaskar et al., 2022, Yang, 2021, Molnar et al., 2019, Wang et al., 2017, Yang et al., 2017, Yang et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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