UAS regulatory sequences drive expression of double-stranded RNA (dsRNA) as a snapback hairpin corresponding to an approximately 500 bp fragment of Pgant3 using a pWIZ vector.
viable, with Scer\GAL4αTub84B.PL
visible, with Scer\GAL4Bx-MS1096
wing blade, with Scer\GAL4Bx-MS1096
Expression of pgant3dsRNA.Scer\UAS.WIZ under the control of Scer\GAL4αTub84B.PL results in complete loss of viability.
Expression of pgant3dsRNA.Scer\UAS.WIZ under the control of Scer\GAL4Bx-MS1096 results in wing blistering.