The rate of proliferation in trypsin-dissociated posterior wing disc cells expressing E2f^{Rb.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E is increased, with a smaller proportion of cells having a G1 DNA content compared to controls.

Expression of E2f^{Rb.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E does not induce apoptosis in the posterior compartment of intact third instar larval wing discs. The same is seen when the wing disc cells are trypsin-dissociated and quantified with flow cytometry.

A smaller proportion of trypsin-dissociated posterior compartment cells from third instar larval wing discs expressing E2f^{Rb.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E are in S phase compared to controls.

As in wild type, very few of the cells posterior to the morphogenetic furrow in eye discs expressing E2f^{Rb.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PU display DNA damage and similar levels of apoptosis are seen compared with controls.