The rate of proliferation in trypsin-dissociated posterior wing disc cells expressing E2f^{PIP-3A-Rb.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E is increased, with a smaller proportion of cells having a G1 DNA content compared to controls.

Expression of E2f^{PIP-3A-Rb.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E induces some apoptosis in the posterior compartment of intact third instar larval wing discs, although the levels are less than when E2f^{PIP-3A.Scer\UAS.P\T.T:Avic\GFP-EGFP} is expressed. A similar increase in apoptosis is seen when the wing disc cells are trypsin-dissociated and quantified using flow cytometry.

A smaller proportion of trypsin-dissociated posterior compartment cells from third instar larval wing discs expressing E2f^{PIP-3A-Rb.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^en-e16E are in S phase compared to controls, however the proportion of cells is significantly higher than in E2f^{Rb.Scer\UAS.P\T.T:Avic\GFP-EGFP}.

The cells posterior to the morphogenetic furrow in eye discs expressing E2f^{Rb.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PU display an increased amount of DNA damage and apoptosis compared with controls.