UAS regulatory sequences drive expression of a mutant E2f1 protein that lacks the N-terminal DNA-binding domain but retains the C-terminal Rbf interaction domain. The construct is tagged at the N-terminal end with Tag:HA.
Expression of E2f336-805.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4en-e16E does not induce apoptosis in the posterior compartment of intact third instar larval wing discs.