UAS regulatory sequences drive expression of an altered kirre protein with the native N-terminal transmembrane domain replaced with Tag:TM(Sema1a) and fused to a C-terminal Tag:FLAG.
Expression of kirreΔN.Scer\UAS.T:TM-Sema-1a,Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4how-24B restores fusion competent myoblast (FCM) attraction and fusion giving rise to a wild-type dorsal muscle DA1 in Df(1)w67k30 mutant embryos.