In addition to the nonsense mutation, the coding region also contains two other alterations that may correspond to pre-existing polymorphisms. These alterations are amino acid replacement C352G and an insertion of six nucleotides, which adds two Ala residues to the stretch of 20 Ala residues spanning amino acids 129-148.
Nucleotide substitution: C?T.
Amino acid replacement: Q204term.
C14505924T
C?T
Q204term | Asx-PA; Q204term | Asx-PB; Q204term | Asx-PC; Q204term | Asx-PD
Q204term
In addition to the nonsense mutation, the coding region also contains two other alterations that may correspond to pre-existing polymorphisms. These alterations are amino acid replacement C352G and an insertion of six nucleotides, which adds two Ala residues to the stretch of 20 Ala residues spanning amino acids 129-148.
Animals containing homozygous clones that cover most of the eye-antennal disc (generated using the eyFLP method) have relatively normal eye morphology, but the antenna has a number of phenotypes suggesting antenna-to-leg transformation coupled with defects in proximodistal axis formation. The basal capsule of the antenna is enlarged and more rounded in shape than normal. The central core of the arista appears bloated, and the organisation of the laterals is abnormal. The position of the arista is abnormal, growing from the edge of the third antennal segment opposite to the contact point between segments 2 and 3. Bracted bristles (normally found on the legs) are seen in the distal portion of the third segment and at the base of the arista. Bracted bristles are not seen on the second antennal segment. Occasionally, the arista appears segmented and lacks the laterals. An outgrowth of ectopic leg-like appendages from the third antennal segment along the domain of dpp expression is seen in 15% of the mutant antennae. In most of these cases there is a single ectopic outgrowth, although a few antennae with up to five outgrowths have been observed. The "original" distal antennal segments retain antennal properties (although the arista has an abnormal morphology) and the ectopic appendages have transverse rows of bracted bristles that suggest a tibial or metatarsal identity.