UAS regulatory sequences drive expression of amino acids 1-597 of Hsap\DISC1 tagged with a Tag:HA epitope.
Expression of Hsap\DISC11-597.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU has no effect on eye structure and morphology in aged adults. Expression under the Scer\GAL4Tab2-201Y driver results in decreased associative olphactory memory performance in third instar larvae compared to controls, but their olphactory as well as gustatory responses are normal.
The axonal branching of γ-neurons in MARCM somatic clones in the adult mushroom body expressing Hsap\DISC11-597.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ey-OK107 is not significantly affected (either the number of branching points or the total branch length) compared to wild-type, the number of dendritic branch points is also reduced, but the number of dendritic claws remains normal.
Expression of Hsap\DISC11-597.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4tub.PU results in reduced total neuromuscular junction bouton area, although their number per junction is not decreased and neither is the number of axonal branching points.
Sleep pattern is not disrupted in flies expressing Hsap\DISC11-597.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Mef2.247.Switch (restricted to the adult stages by feeding flies RU486 from 3-6 days onwards).