FlyBase Allele Report: Dmel\orb2[ΔQΔAGFP]

General Information

Symbol

Dmel\orb2^ΔQΔAGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0280510

Feature type

allele

Associated gene

Dmel\orb2

Associated Insertion(s)

TI{TI}orb2^ΔQΔAGFP

Carried in Construct

Key Links

Allele class

Mutagen

phiC31 integrase

Nature of the Allele

Allele class

Mutagen

phiC31 integrase

(Krüttner et al., 2012)

Progenitor genotype

orb2^attP

(Krüttner et al., 2012)

Associated Insertion(s)

TI{TI}orb2^ΔQΔAGFP

Cytology

Description

A modified orb2 genomic fragment has been inserted into the attP site present in orb2^attP via phiC31-mediated recombination. The resulting allele contains an insertion of a single nucleotide in the A isoform specific exon, which disrupts the the orb2A isoform but leaves the orb2B isoform (which is tagged at the C-terminal end with GFP) intact. In addition, the Q domain has been deleted. A single mFRT11 site is present downstream of the orb2 coding sequence, and single attL and attR sites (generated during the recombination process) are also present.

(Krüttner et al., 2012)

Allele components

Component

Use(s)

Tagged with

GFP

green fluorescent protein

Also carries

mFRT11

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | adult stage

(Krüttner et al., 2012)

abnormal courtship behavior | adult stage (with orb2^ΔBGFP)

(Krüttner et al., 2012)

abnormal learning | adult stage

(Krüttner et al., 2012)

abnormal learning | adult stage (with orb2^ΔBGFP)

(Krüttner et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous orb2^{ΔQΔA.T:Avic\GFP} male flies have almost no long term 24 hour memory when exposed to a courtship conditioning paradigm. Unlike wild type flies, males that experience futile courtship of mated and therefore unreceptive females fail to suppress their courtship towards other mated females. Short term 1 hour memory is comparable to wild type.

orb2^{ΔQΔA.T:Avic\GFP}/orb2^{ΔB.T:Avic\GFP} male flies show defects in long term 24 hour memory when exposed to a courtship conditioning paradigm, and these are similar in severity to orb2^{ΔB.T:Avic\GFP}/orb2^{ΔA.T:Avic\GFP} males. Unlike wild type flies, males that experience futile courtship of mated and therefore unreceptive females often fail to suppress their courtship towards other mated females.

orb2^{ΔQΔA.T:Avic\GFP}/orb2^{RBDΔB.T:Avic\GFP} male flies have a long term 24 hour memory comparable to wild type when exposed to a courtship conditioning paradigm. Males that experience futile courtship of mated and therefore unreceptive females suppress their courtship towards other mated females.

(Krüttner et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

orb2^ΔAΔQGFP

(Krüttner et al., 2012)

orb2^ΔQDA

(Krüttner et al., 2012)

orb2^{ΔQΔA.T:Avic\GFP}

orb2^ΔQΔA

(Krüttner et al., 2012)

orb2^ΔQΔAGFP

(Krüttner et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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