FlyBase Allele Report: Dmel\Fancd2[RNAi.UAS]

General Information

Symbol

Dmel\Fancd2^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0281761

Feature type

allele

Associated gene

Dmel\Fancd2

Associated Insertion(s)

Carried in Construct

P{UAS-Fancd2.RNAi}

Also Known As

FANCD2 RNAi

Key Links

Transgenic product class

RNAi_reagent

(Marek and Bale, 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Marek and Bale, 2006)

Mutagen

in vitro construct

(Marek and Bale, 2006)

Progenitor genotype

Carried in construct

P{UAS-Fancd2.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of an inverse repeat targeting the second exon of Fancd2 (bases 356-860).

(Marek and Bale, 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Fancd2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Fanconi anemia

CEA

(Marek and Bale, 2006)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye | chemical conditional, with Scer\GAL4^ey.PU

(Marek and Bale, 2006)

Detailed Description

Statement

Reference

Flies expressing Fancd2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PU have no observable morphological defects. When exposed to four different cross-linking agents (diepoxybutane, mitomycin C, cisplatinum and nitrogen mustard) a greater proportion of flies have small, rough eyes compared to wild type controls. Ectopic growths are also seen. This response is not observed in response to the alkylating agent methylmethanesulfanate or in response to radiation treatment.

In contrast to controls, expression of Fancd2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act.PU results in chromosome nondisjunction in larval neuroblasts. The rate of chromosome fusions is enhanced in response to cisplatinum treatment, in particular the frequency with which chromosome four is fused with chromosome two. No difference in relative telomere length is seen in flies expressing Fancd2^{dsRNA.Scer\UAS} compared to controls.

Unlike in wild type, cells in the brain lobes of flies expressing Fancd2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act.PU continue to progress through the S-phase checkpoint following exposure to ionising irradiation.

(Marek and Bale, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Fancd2^RNAi.UAS, Scer\GAL4^ey.PU has visible | chemical conditional phenotype, non-enhanceable by Mnn1^UASp.cBa, Scer\GAL4^ey.PU

(Marek et al., 2008)

Fancd2^RNAi.UAS, Scer\GAL4^ey.PU has visible | chemical conditional phenotype, non-enhanceable by Mnn1^e200

(Marek et al., 2008)

Fancd2^RNAi.UAS, Scer\GAL4^ey.PU has visible | chemical conditional phenotype, non-enhanceable by Fancl^RNAi.UAS, Scer\GAL4^ey.PU

(Marek and Bale, 2006)

Fancd2^RNAi.UAS, Scer\GAL4^ey.PU has neoplasia | chemical conditional phenotype, non-enhanceable by Fancl^RNAi.UAS, Scer\GAL4^ey.PU

(Marek and Bale, 2006)

Fancd2^RNAi.UAS has chemical sensitive phenotype, non-enhanceable by Fancl^RNAi.UAS/Scer\GAL4^ey.PU

(Marek and Bale, 2006)

NOT suppressed by

Statement

Reference

Fancd2^RNAi.UAS, Scer\GAL4^ey.PU has visible | chemical conditional phenotype, non-suppressible by Mnn1^UASp.cBa, Scer\GAL4^ey.PU

(Marek et al., 2008)

Fancd2^RNAi.UAS, Scer\GAL4^ey.PU has visible | chemical conditional phenotype, non-suppressible by Mnn1^e200

(Marek et al., 2008)

Other

Statement

Reference

Fancd2^RNAi.UAS, Scer\GAL4^ey.PU, wts^x1/wts[+] has chemical sensitive phenotype

(Marek et al., 2008, Marek and Bale, 2006)

Fancd2^RNAi.UAS, Scer\GAL4^ey.PU, wts^x1/wts[+] has neoplasia | conditional phenotype

(Marek et al., 2008, Marek and Bale, 2006)

Fancd2^RNAi.UAS, Scer\GAL4^ey.PU, wts^x1/wts[+] has visible | chemical conditional phenotype

(Marek et al., 2008, Marek and Bale, 2006)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Fancd2^RNAi.UAS, Scer\GAL4^ey.PU has eye | chemical conditional phenotype, non-enhanceable by Mnn1^UASp.cBa, Scer\GAL4^ey.PU

(Marek et al., 2008)

Fancd2^RNAi.UAS, Scer\GAL4^ey.PU has eye | chemical conditional phenotype, non-enhanceable by Mnn1^e200

(Marek et al., 2008)

Fancd2^RNAi.UAS, Scer\GAL4^ey.PU has eye | chemical conditional phenotype, non-enhanceable by Fancl^RNAi.UAS, Scer\GAL4^ey.PU

(Marek and Bale, 2006)

NOT suppressed by

Statement

Reference

Fancd2^RNAi.UAS, Scer\GAL4^ey.PU has eye | chemical conditional phenotype, non-suppressible by Mnn1^UASp.cBa, Scer\GAL4^ey.PU

(Marek et al., 2008)

Fancd2^RNAi.UAS, Scer\GAL4^ey.PU has eye | chemical conditional phenotype, non-suppressible by Mnn1^e200

(Marek et al., 2008)

Other

Statement

Reference

Fancd2^RNAi.UAS, Scer\GAL4^ey.PU, wts^x1/wts[+] has eye | chemical conditional phenotype

(Marek et al., 2008, Marek and Bale, 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Fancd2^{dsRNA.Scer\UAS} in the eyes under the control of Scer\GAL4^ey.PU enhances the rate of eye tumor formation in wts^x1/+ mutant flies treated with nitrogen mustard.

Expression of Mnn1^{Scer\UAS.P\T.cBa} does not alter the rough eye phenotype seen in flies expressing Fancd2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PU in response to the cross-linking agent cisplatinum.

The presence of homozygous Mnn1^e200 does not alter the rough eye phenotype seen in flies expressing Fancd2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PU in response to the cross-linking agent cisplatinum.

(Marek et al., 2008)

Expression of Fancd2^{dsRNA.Scer\UAS} in the eyes under the control of Scer\GAL4^ey.PU in a wts^x1/+ mutant background does not enhance the rate of eye tumor formation compared to controls in normal conditions. However the number of eye tumors is significantly increased when flies are treated with either nitrogen mustard or cisplatinum. This increase is restricted to eye tumors; the rate of tumours in other anatomical locations is similar to controls.

Expression of Fancl^{dsRNA.Scer\UAS} is unable to enhance the severity of the rough eye phenotype seen when Fancd2^{dsRNA.Scer\UAS} is expressed alone. The proportion of flies that display a rough eye phenotype is similar across both of the single mutants and the double mutant.

(Marek and Bale, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Fancd2^RNAi.UAS

Fancd2^{dsRNA.Scer\UAS}

Fancd2^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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