FB2026_03 , released September 17, 2026
Allele: Dmel\CaMKIIUAS.TimeSTAMPt
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General Information
Symbol
Dmel\CaMKIIUAS.TimeSTAMPt
Species
D. melanogaster
Name
FlyBase ID
FBal0282354
Feature type
allele
Associated gene
Dmel\CaMKII
Associated Insertion(s)
Carried in Construct
P{UAS-HSV-TimeSTAMPt-HA-CaMKII}
Key Links
Transgenic product class
wild_type
(Lin et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Lin et al., 2008)
Mutagen
in vitro construct
(Lin et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-HSV-TimeSTAMPt-HA-CaMKII}
Cytology
Description

UAS regulatory sequences drive expression of a coding sequence that consists of an HSV epitope tag, a TimeSTAMPt cassette, a Tag:HA tag and CaMKII. The CaMKII protein is constitutively tagged with Tag:HA, but the presence of the TimeSTAMPt cassette means that tagging of CaMKII with the HSV epitope tag is drug-dependent, requiring the presence of a specific inhibitor (BILN-2061). In the absence of BILN-2061, the TimeSTAMPt cassette catalyzes its own cleavage from the protein sequence, and thus the upstream HSV epitope tag is removed from the CaMKII protein. In the presence of BILN-2061 this cleavage is inhibited, and thus any CaMKII protein newly synthesized in the presence of the inhibitor is tagged with the HSV epitope tag in addition to Tag:HA.

(Lin et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
CaMKII
Tagged with
Tag:HA
TimeSTAMPt
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CaMKIIScer\UAS.TimeSTAMPt
      CaMKIIUAS.TimeSTAMPt
      Name Synonyms
      Secondary FlyBase IDs
        References (1)