FlyBase Allele Report: Hsap\TTR[wt.UAS.Tag:HA]

General Information

Symbol

Hsap\TTR^{wt.UAS.Tag:HA}

Species

H. sapiens

Name

FlyBase ID

FBal0283625

Feature type

allele

Associated gene

Hsap\TTR

Associated Insertion(s)

Carried in Construct

P{UAS-TTR.wt}

Key Links

Transgenic product class

wild_type

(Berg et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Berg et al., 2009)

Mutagen

in vitro construct

(Berg et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-TTR.wt}

Cytology

Description

UASt regulates expression of wild type Hsap\TTR, codon optimised for Drosophila, and containing the human secretory signal peptide for Hsap\TTR and a C-terminal Tag:HA tag.

(Berg et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\TTR

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of hereditary systemic amyloidosis 1

CEA

(Berg et al., 2009)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^elav-C155

(Berg et al., 2009)

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Berg et al., 2009)

short lived, with Scer\GAL4^elav-C155

(Berg et al., 2009)

Phenotype Manifest In

adult brain, with Scer\GAL4^elav-C155

(Berg et al., 2009)

eye | progressive, with Scer\GAL4^GMR.PU

(Berg et al., 2009)

ommatidium | progressive, with Scer\GAL4^GMR.PU

(Berg et al., 2009)

Detailed Description

Statement

Reference

Scer\GAL4^elav-C155-mediated expression of Hsap\TTR^{wt.Scer\UAS.T:Ivir\HA1} reduces median lifespan to 28 days, compared to 32 days in controls.

Scer\GAL4^elav-C155-mediated expression of Hsap\TTR^{wt.Scer\UAS.T:Ivir\HA1} results in reduced climbing activity compared to controls.

Scer\GAL4^elav-C155-mediated expression of Hsap\TTR^{wt.Scer\UAS.T:Ivir\HA1} generates an increased number of vacuoles in the brain of 20 day-old flies compared to controls. Unlike controls, these brains also show some large vacuoles.

By day 25 after eclosion, Scer\GAL4^GMR.PU-mediated expression of Hsap\TTR^{wt.Scer\UAS.T:Ivir\HA1} results in a mild eye phenotype - it is overtly normal, but there are small areas of fibrillar deposition and the ommatidia show mild curly morphology, though there is no vacuolisation.

(Berg et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\TTR^{wt.Scer\UAS.T:Ivir\HA1}

Hsap\TTR^{wt.UAS.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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