UAS regulatory sequences drive expression of Delta coding sequences carrying a K742R amino acid replacement, and tagged at the C-terminal with Tag:V5 and Tag:polyHis.
Expression of DlK742R.Scer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of the ubiquitous driver Scer\GAL4mat.αTub67C.T:Hsim\VP16 fails to restore lateral inhibition N signalling in DlRevF10 SerRX106 mutant clones.