Excision of the FRT-w cassette from progenitor AxnFRT.UAS.Tag:FLAG allows the UAS sequences to drive expression of full-length Axn fused with N-terminal 6xTag:FLAG.
Transgenic flies co-expressing Scer\GAL4ptc-559.1>AxnScer\UAS.T:Zzzz\FLAG and AxnαTub84B.T:Zzzz\FLAG in addition to endogenous wild-type Axn show no visible defects in the bristle pattern or at the wing margin.