FlyBase Allele Report: Ggal\MLCK[CaMBP4.UAS.Tag:MYC]

General Information

Symbol

Ggal\MLCK^{CaMBP4.UAS.Tag:MYC}

Species

G. gallus

Name

FlyBase ID

FBal0285613

Feature type

allele

Associated gene

Ggal\MLCK

Associated Insertion(s)

Carried in Construct

P{UAS-CaMBP4}

Also Known As

UAS-CaMBP4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Weislogel et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-CaMBP4}

Cytology

Description

UASt regulatory sequences drive expression of a blocker of nuclear Ca[2+] signaling. This consists of four copies of the M13 peptide from Ggal\MLCK, each of which contains an N-terminal cryptic nuclear localization signal which is sufficient for nuclear localisation. In addition, a Tag:MYC epitope has been inserted at the C-terminal end of the coding sequence.

(Weislogel et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ggal\MLCK

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Mahoney et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory, with Scer\GAL4^ey-OK107

(Weislogel et al., 2013)

abnormal memory, with Scer\GAL4^Tab2-201Y

(Weislogel et al., 2013)

abnormal memory | heat sensitive, with Scer\GAL4^hs.PU

(Weislogel et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies expressing Ggal\MLCK^{CaMBP4.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^hs.PU during conditioning (expression induced by a 30 minute heat shock 12-18 hours prior to conditioning) show no significant impairment in short-term memory, middle-term memory or anesthesia-resistant memory, but show a significant impairment in long-term memory, in an olfactory avoidance conditioning assay.

Flies expressing Ggal\MLCK^{CaMBP4.Scer\UAS.T:Hsap\MYC} under the control of either Scer\GAL4^ey-OK107 or Scer\GAL4^Tab2-201Y for 5 to 6 days prior to testing (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to prevent expression during development) do not show defects in short-term memory, but show a significant impairment in long-term memory, in an olfactory avoidance conditioning assay.

Flies expressing Ggal\MLCK^{CaMBP4.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^c739 for 5 to 6 days prior to testing (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to prevent expression during development) do not show defects in short-term memory or in long-term memory in an olfactory avoidance conditioning assay.

(Weislogel et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ggal\MLCK^{CaMBP4.Scer\UAS.T:Hsap\MYC}

Ggal\MLCK^{CaMBP4.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close