UASt regulatory sequences drive expression of JIL-1 containing a mutation in each of the two kinase domains (amino acid replacements K293A and K652A respectively). The protein is tagged at the C-terminal end with Tag:V5.
JIL-1KDI-KDIImut.UAS.Tag:V5 partially rescues JIL-1z2
JIL-1KDI-KDIImut.Scer\UAS.T:SV5\V5 fails to rescue polytene chromosome morphology in JIL-1z2 mutant animals. Rescue of adult viability is 22.9%.