FB2025_05 , released December 11, 2025
Allele: Dmel\mir-314UAS.mCherry
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General Information
Symbol
Dmel\mir-314UAS.mCherry
Species
D. melanogaster
Name
FlyBase ID
FBal0287593
Feature type
allele
Associated gene
Dmel\mir-314
Associated Insertion(s)
Carried in Construct
P{UAS-mCherry-mir-314}
Key Links
Transgenic product class
wild_type
(Nesler et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Nesler et al., 2013)
Mutagen
in vitro construct
(Nesler et al., 2013)
Progenitor genotype
Carried in construct
P{UAS-mCherry-mir-314}
Cytology
Description

UAS regulatory sequences drive expression of the mCherry version of Disc\RFP in which the pri-miRNA sequence of mir-314 (consists of a genomic fragment which includes the miRNA hairpin and approximately 200bp both upstream and downstream of it) has been inserted into the 3'UTR sequence.

(Nesler et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
mir-314
Tagged with
mCherry
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of mir-314Scer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4futsch-C380 has no effect on the increase in "ghost bouton" number (axon terminal extensions with synaptic vesicles but lacking active zones and postsynaptic structures) per neuromuscular junction caused by spaced high K[+] depolarization.

      Total bouton number at the neuromuscular junction is not significantly different from wild type in larvae expressing mir-314Scer\UAS.T:Disc\RFP-mCherry under the control of Scer\GAL4futsch-C380.

      (Nesler et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      mir-314Scer\UAS.T:Disc\RFP-mCherry
      mir-314UAS.mCherry
      Name Synonyms
      Secondary FlyBase IDs
        References (1)