FlyBase Allele Report: Hsap\LRRK2[WT.UAS.Tag:MYC]

General Information

Symbol

Hsap\LRRK2^{WT.UAS.Tag:MYC}

Species

H. sapiens

Name

FlyBase ID

FBal0288350

Feature type

allele

Associated gene

Hsap\LRRK2

Associated Insertion(s)

Carried in Construct

P{UAS-LRRK2.WT.myc}

Also Known As

LRRK2-WT

Key Links

Transgenic product class

wild_type

(Ng et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ng et al., 2009)

Mutagen

in vitro construct

(Ng et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-LRRK2.WT.myc}

Cytology

Description

UASt regulates expression of wild type Hsap\LRRK2 tagged with Tag:MYC at the C terminus.

(Ng et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\LRRK2

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

short lived, with Scer\GAL4^elav.PU

(Angeles et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Hsap\LRRK2^{WT.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Ddc.PL does not result in climbing defects or loss of dopaminergic neurons, as compared to controls.

(Ng et al., 2017)

Adults expressing Hsap\LRRK2^{WT.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav.PU present a decreased lifespan compared to controls.

(Angeles et al., 2014)

Expression of Hsap\LRRK2^{WT.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^how-24B has no effect on mitochondrial morphology in the flight muscles. Climbing behaviour is also normal.

(Ng et al., 2012)

Scer\GAL4^elav.PU-mediated expression of Hsap\LRRK2^{WT.Scer\UAS.T:Hsap\MYC} does not appear to compromise the overall anatomical integrity of the fly’s brain up to 60 d after eclosion.

Scanning electron microscope analysis reveals no apparent eye abnormalities in Scer\GAL4^GMR.PU Hsap\LRRK2^{WT.Scer\UAS.T:Hsap\MYC} flies at 20 d after eclosion. Similar observations are made when these flies are aged to 60 d.

All the DA neuronal clusters, including PPM 1 and 3, remain unaffected in flies expressing Hsap\LRRK2^{WT.Scer\UAS.T:Hsap\MYC} via Scer\GAL4^Ddc.PL relative to age-matched control flies.

Flies expressing Hsap\LRRK2^{WT.Scer\UAS.T:Hsap\MYC} via Scer\GAL4^Ddc.PL record similar climbing scores as controls.

There is no accelerated loss of DA neurons of rotenone-treated flies expressing Hsap\LRRK2^{WT.Scer\UAS.T:Hsap\MYC} via Scer\GAL4^Ddc.PL, compared to controls.

(Ng et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsap\LRRK2^{WT.UAS.Tag:MYC}, Scer\GAL4^elav.PU has short lived phenotype, suppressible | partially by Hsap\PRDX3^UAS.cAa, Scer\GAL4^elav.PU

(Angeles et al., 2014)

Suppressor of

Statement

Reference

Hsap\LRRK2^{WT.UAS.Tag:MYC}, Scer\GAL4^elav.PU is a suppressor | partially of short lived phenotype of Hsap\PRDX3^UAS.cAa, Scer\GAL4^elav.PU

(Angeles et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\LRRK2^{WT.Scer\UAS.T:Hsap\MYC} partially suppresses the decreased lifespan induced by the expression of Hsap\PRDX3^Scer\UAS.cAa under the control of Scer\GAL4^elav.PU.

(Angeles et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\LRRK2^{WT.Scer\UAS.T:Hsap\MYC}

Hsap\LRRK2^{WT.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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